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Peripheral blood lymphocyte proviral DNA predicts neurocognitive impairment in clade C HIV

dc.contributor.authorRuhanya, Vurayaien_ZA
dc.contributor.authorJacobs, Graeme Brendonen_ZA
dc.contributor.authorNyandoro, Georgeen_ZA
dc.contributor.authorPaul, Robert H.en_ZA
dc.contributor.authorJoska, John A.en_ZA
dc.contributor.authorSeedat, Sorayaen_ZA
dc.contributor.authorGlashoff, Richard Helmuthen_ZA
dc.contributor.authorEngelbrecht, Susanen_ZA
dc.date.accessioned2023-01-19T07:35:10Zen_ZA
dc.date.available2023-01-19T07:35:10Zen_ZA
dc.date.issued2020-07en_ZA
dc.identifier.citationRuhanya, V. et al. 2020. Peripheral blood lymphocyte proviral DNA predicts neurocognitive impairment in clade C HIV. Journal of NeuroVirology, 26:920–928, doi:10.1007/s13365-020-00882-9.en_ZA
dc.identifier.issn1538-2443 (online)en_ZA
dc.identifier.issn1355-0284 (print)en_ZA
dc.identifier.otherdoi:10.1007/s13365-020-00882-9en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/126248en_ZA
dc.descriptionCITATION: Ruhanya, V. et al. 2020. Peripheral blood lymphocyte proviral DNA predicts neurocognitive impairment in clade C HIV. Journal of NeuroVirology, 26:920–928, doi:10.1007/s13365-020-00882-9.en_ZA
dc.descriptionThe original publication is available at https://link.springer.comen_ZA
dc.description.abstractIt is not known if proviral DNA in the periphery corresponds to cognitive status in clade C as it does in clade B and recombinant forms. A cross-sectional study was conducted on participants investigated for HIV-associated neurocognitive impairment in South Africa. HIV-1 proviral DNA was quantified using a PCR assay targeting a highly conserved HIV-1 LTR-gag region. Fifty-four (36.7%) participants were cognitively impaired and 93 (63.3%) were not impaired. Forty-three (79.6%) of the cognitively impaired participants were female and 11 (20.4%) were male. There was no significant age difference between cognitively impaired and unimpaired participants (p = 0.42). HIV-1 DNA in cognitively impaired PLWH was significantly higher than in cognitively normal individuals (p = .016). Considering impaired participants, lymphocyte HIV-1 DNA was significantly higher in males than females (p = 0.02). There was a modest positive correlation between lymphocyte HIV-1 DNA and global deficit scores (GDS) r = 0.176; p = 0.03). The two measures of viral load, lymphocyte HIV-1 DNA copies/million and plasma RNA copies/ml, were positively correlated (r = 0.39; p < .001). After adjusting for other covariates, age, sex, treatment status, and the interactions between impairment and treatment, the multivariate regression showed association between proviral load and neurocognitive impairment; omega effect size was 0.04, p value = 0.010. The burden of HIV-1 peripheral blood lymphocyte proviral DNA corresponds to neurocognitive impairment among individuals infected with clade C disease. Therefore, therapeutic strategies to reduce the HIV-1 proviral DNA reservoir in lymphocytes may improve neurocognitive outcomes in PLWH.en_ZA
dc.description.sponsorshipPoliomyelitis Research Foundation (PRF)en_ZA
dc.description.sponsorshipNational Research Foundation (NRF)en_ZA
dc.description.sponsorshipSouth African Medical Research Council (SAMRC)en_ZA
dc.format.extent9 pagesen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherSpringeren_ZA
dc.subjectBlood lymphocytesen_ZA
dc.subjectHIV infectionsen_ZA
dc.subjectLymphocyteen_ZA
dc.subjectNeurocognitive disordersen_ZA
dc.subjectHIV infections -- Complicationsen_ZA
dc.titlePeripheral blood lymphocyte proviral DNA predicts neurocognitive impairment in clade C HIVen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's versionen_ZA
dc.rights.holderAuthors retain copyrighten_ZA


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