Hormonal profile and reproductive parameters of pre-vitellogenic mozambique tilapia (oreochromis mossambicus) on pawpaw (carica papaya) seed meal

Abstract

The use of phytochemicals to control precocious maturation and indiscriminate spawning among tilapia species is an important issue in aquaculture. This study investigated the influence of pawpaw (Carica papaya) seed meal on the reproductive performance, and the sex hormone profile of sexually immature Mozambique tilapia (Oreochromis mossambicus). Pawpaw seed meal (PSM) were included in a tilapia commercial diet (basal diet) at an inclusion level of 0 PSM, 10 PSM and 30 PSM g/kg of the basal diet (BD), respectively, and fed to immature O. mossambicus for a period of 30 and 60 days, respectively. Enzyme-linked immune-sorbent assay (ELISA) procedures were used to quantify the plasma levels of 17β-estradiol and 11-ketotestosterone. The inclusion of PSM did not affect the growth and survival rate of the O. mossambicus. The plasma levels of 11-ketotestosterone did not differ among the treatment groups. The 17β-estradiol levels of female fish that received 30 PSM g/kg BD for 60 days were significantly lower (p < 0.05) than the levels reported for females that were not fed the PSM. In females, the PSM reduced the gonad weight, GSI, fecundity and egg diameter of fish. The study concludes that pawpaw seed meal affected the reproductive function of the female O. mossambicus though the changes were reversible.