Identification of a functional TATA element in the STA2 glucoamylase gene promoter from Saccharomyces cerevisiae

Date
1998
Authors
Vivier M.A.
Pretorius I.S.
Journal Title
Journal ISSN
Volume Title
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Abstract
Accurate transcription by RNA polymerase II is usually dependent on the presence of a TATA element, and/or an initiator element, in the promoters of protein-encoding genes. The STA1-3 genes, encoding three glucoamylase isozymes (Sta1p, Sta2p and Sta3p, respectively) responsible for starch hydrolysis in the yeast Saccharomyces cerevisiae, have been shown to contain long and complex promoters with several regulatory regions. These promoters are also virtually identical to the yeast MUC1 gene promoter; this gene encodes a mucin-like protein and is evolutionary linked to, and transcriptionally co-regulated with, STA1-3. The STA1-3 genes contain two putative TATA sequences, one conforming to the typical TATA box sequence, TATAAA, and another with the sequence of TATAAT. Here we present a study into the functional relevance of these putative TATA sequences and their effects on the transcription of the STA2 gene (as a representative model of the STA1-3 multigene family) and, by analogy, the MUC1 gene. We show that the TATAAA motif is the functional TATA box for STA2 and influences transcript levels, transcript initiation sites, and glucoamylase activities.
Description
Keywords
glucan 1,4 alpha glucosidase, isoenzyme, mucin, rna polymerase ii, starch, article, controlled study, deletion mutant, enzyme activity, fungal genetics, gene expression, gene mapping, genetic transcription, hydrolysis, multigene family, nonhuman, northern blotting, priority journal, promoter region, regulator gene, saccharomyces cerevisiae, tata box, transcription initiation, Enzyme Activation, Gene Deletion, Glucan 1,4-alpha-Glucosidase, Isoenzymes, Peptide Chain Initiation, Translational, Polymerase Chain Reaction, Promoter Regions (Genetics), Saccharomyces cerevisiae, TATA Box, Transcription, Genetic, Saccharomyces cerevisiae
Citation
Current Genetics
33
1