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Biofilm dynamics : linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions

dc.contributor.authorKlopper, Kyle Brenten_ZA
dc.contributor.authorDe Witt, Riaan N.en_ZA
dc.contributor.authorBester, Elannaen_ZA
dc.contributor.authorDicks, Leon Milner Theodore, 1961-en_ZA
dc.contributor.authorWolfaardt, Gideon M.en_ZA
dc.date.accessioned2020-11-09T09:34:11Z
dc.date.available2020-11-09T09:34:11Z
dc.date.issued2020
dc.identifier.citationKlopper, K. B., et al. Biofilm dynamics : linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions. npj Biofilms and Microbiomes, 6:42, doi:10.1038/s41522-020-00153-9en_ZA
dc.identifier.issn2055-5008 (online)
dc.identifier.otherdoi:10.1038/s41522-020-00153-9
dc.identifier.urihttp://hdl.handle.net/10019.1/108923
dc.descriptionCITATION: Klopper, K. B., et al. Biofilm dynamics : linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions. npj Biofilms and Microbiomes, 6:42, doi:10.1038/s41522-020-00153-9.
dc.descriptionThe original publication is available at https://www.nature.com
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund
dc.description.abstractThe tools used to study biofilms generally involve either destructive, end-point analyses or periodic measurements. The advent of the internet of things (IoT) era allows circumvention of these limitations. Here we introduce and detail the development of the BioSpec; a modular, nondestructive, real-time monitoring system, which accurately and reliably track changes in biofilm biomass over time. The performance of the system was validated using a commercial spectrophotometer and produced comparable results for variations in planktonic and sessile biomass. BioSpec was combined with the previously developed carbon dioxide evolution measurement system (CEMS) to allow simultaneous measurement of biofilm biomass and metabolic activity and revealed a differential response of these interrelated parameters to changing environmental conditions. The application of this system can facilitate a greater understanding of biofilm mass–function relationships and aid in the development of biofilm control strategies.en_ZA
dc.description.urihttps://www.nature.com/articles/s41522-020-00153-9
dc.format.extent10 pages : illustrationsen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherNature Research
dc.subjectBiofilmsen_ZA
dc.subjectWater -- Microbiologyen_ZA
dc.subjectBiofilms -- Microbiologyen_ZA
dc.subjectMicrobial aggregationen_ZA
dc.subjectAggregation, Microbialen_ZA
dc.subjectMicrobial ecologyen_ZA
dc.subjectMicroorganisms -- Aggregationen_ZA
dc.subjectEnvironmental microbiologyen_ZA
dc.subjectCarbon dioxide -- Measurementen_ZA
dc.subjectCarbon Dioxide Evolution Measurement Systems (CEMS)en_ZA
dc.subjectSpectrophotometeren_ZA
dc.titleBiofilm dynamics : linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditionsen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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