ITEM VIEW

Nitrogen-fixing bacteria and Oxalis – evidence for a vertically inherited bacterial symbiosis

dc.contributor.authorJooste, Michelleen_ZA
dc.contributor.authorRoets, Francoisen_ZA
dc.contributor.authorMidgley, Guy F.en_ZA
dc.contributor.authorOberlander, Kenneth C.en_ZA
dc.contributor.authorDreyer, Leanne L.en_ZA
dc.date.accessioned2019-10-28T05:25:22Z
dc.date.available2019-10-28T05:25:22Z
dc.date.issued2019-10-23
dc.identifier.citationJooste, M., et al. 2019. Nitrogen-fixing bacteria and Oxalis – evidence for a vertically inherited bacterial symbiosis. BMC Plant Biology, 19:441, doi:10.1186/s12870-019-2049-7en_ZA
dc.identifier.issn1471-2229 (online)
dc.identifier.otherdoi:10.1186/s12870-019-2049-7
dc.identifier.urihttp://hdl.handle.net/10019.1/106735
dc.descriptionCITATION: Jooste, M., et al. 2019. Nitrogen-fixing bacteria and Oxalis – evidence for a vertically inherited bacterial symbiosis. BMC Plant Biology, 19:441, doi:10.1186/s12870-019-2049-7.en_ZA
dc.descriptionThe original publication is available at https://bmcplantbiol.biomedcentral.comen_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.description.abstractBackground: Plant-endophyte symbioses often revolve around nitrogen metabolism, and involve varying degrees of intimacy. Although evidence for vertical inheritance of nitrogen-fixing endophytic bacteria is increasing, it is confined mostly to crop plants, and to date no such system has been reported for geophytes. Methods: Bacterial endophytes associated with Oxalis, the most species-rich geophytic genus form the Cape Flora in southern Africa was studied. Culturable endophytes were isolated from surface-sterilized vegetative and reproductive plant organs for six host species at three locations. Colonies of microbes on various artificial media were morphotyped, enumerated and identified using sequence data. Filter exclusion experiments were conducted to determine if endophytes were vertically transmitted to seeds, determine if mucilage plays a role to actively attract microbes from the soil and to assess microbial richness isolated from the mucilage of Oxalis seedlings. Fluorescent microscopy was implemented in order to visualize endophytic bacteria in cryo-sectioned seeds. Results: Evidence for a novel, vertically transmitted symbiosis was reported. Communities of nitrogen-fixing and plant growth-promoting Bacillus endophytes were found to associate with selected Oxalis hosts from nitrogen-deficient environments of the Cape. Bacillus endophytes were ubiquitous and diverse across species and plant bodies, and were prominent in seeds. Three common nitrogen-fixing Bacillus have known oxalotrophic properties and appear to be housed inside specialised cavities (containing oxalates) within the plant body and seeds. Conclusions: The discovery of vertical transmission and potential benefits to both host and endophyte suggest a particularly tight mutualism in the Oxalis-endophyte system. This discovery suggests unexpected ways in which geophytes might avoid nitrogen deficiency, and suggest that such symbioses are more common than previously expected.en_ZA
dc.description.urihttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2049-7
dc.format.extent10 pages : illustrations, mapen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherBMC (part of Springer Nature)en_ZA
dc.subjectNitrogen -- Fixationen_ZA
dc.subjectPlant-endophyte symbiosesen_ZA
dc.subjectGeophytesen_ZA
dc.subjectPlant-microbial interactionsen_ZA
dc.subjectEndophytic bacteriaen_ZA
dc.titleNitrogen-fixing bacteria and Oxalis – evidence for a vertically inherited bacterial symbiosisen_ZA
dc.typeArticleen_ZA
dc.date.updated2019-10-27T06:29:04Z
dc.description.versionPublisher's versionen_ZA
dc.rights.holderAuthors retain copyrighten_ZA


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

ITEM VIEW