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Measuring antigen-specific responses in Mycobacterium bovis-infected warthogs (Phacochoerus africanus) using the intradermal tuberculin test

dc.contributor.authorRoos, Eduard O.en_ZA
dc.contributor.authorOlea-Popelka, Franciscoen_ZA
dc.contributor.authorBuss, Peter E.en_ZA
dc.contributor.authorHausler, Guyen_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorParsons, Sven D. C.en_ZA
dc.contributor.authorDe Klerk-Lorist, Lin-Marien_ZA
dc.contributor.authorMiller, Michele Annen_ZA
dc.date.accessioned2018-11-29T14:01:29Z
dc.date.available2018-11-29T14:01:29Z
dc.date.issued2018
dc.identifier.citationRoos, E. O., et al. 2018. Measuring antigen-specific responses in Mycobacterium bovis-infected warthogs (Phacochoerus africanus) using the intradermal tuberculin test. BMC Veterinary Research, 14:360, doi:10.1186/s12917-018-1685-8en_ZA
dc.identifier.issn1746-6148 (online)
dc.identifier.otherdoi:10.1186/s12917-018-1685-8
dc.identifier.urihttp://hdl.handle.net/10019.1/104752
dc.descriptionCITATION: Roos, E. O., et al. 2018. Measuring antigen-specific responses in Mycobacterium bovis-infected warthogs (Phacochoerus africanus) using the intradermal tuberculin test. BMC Veterinary Research, 14:360, doi:10.1186/s12917-018-1685-8.en_ZA
dc.descriptionThe original publication is available at https://bmcvetres.biomedcentral.comen_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.description.abstractBackground: Bovine tuberculosis (bTB) caused by Mycobacterium bovis has previously been diagnosed in warthogs and infection can be highly prevalent (> 30%) in endemic areas. Thus, warthogs could potentially be an important species to consider as sentinels for disease surveillance. However, disease surveillance is dependent on availability of accurate diagnostic assays and only a few diagnostic tests have been investigated for warthogs. Furthermore, the tests that have been used in this species require laboratory equipment and trained personnel to obtain results. Therefore, this study investigated the use of the intradermal tuberculin test (ITT) to screen warthogs for bTB, which can be done with minimal equipment and under field conditions by most veterinarians and other qualified professionals. Changes in skin fold thickness measurements at the bovine purified protein derivative (PPD) administration site, between 0 and 72 h, were compared with differential changes between the bovine and avian PPD sites, for 34 warthogs, to evaluate the performance when different interpretation criteria for the ITT was used. Results: Using an increase of 1.8 mm or more at the bovine PPD site as a cut-off for positive responders, 69% of 16 M. bovis culture-positive warthogs had a positive test result, with 100% of the 18 culture-negative warthogs considered as test negative. When a differential of 1.2 mm or more in skin fold thickness at the bovine PPD compared to the avian PPD site was used as a cut-off for the comparative ITT, 81% of culture-positive warthogs were considered as test positive, with 100% of culture-negative warthogs considered as test negative. Conclusion: The findings in this study suggest that the ITT is a promising tool to use when screening warthogs for M. bovis infection.en_ZA
dc.description.urihttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1685-8
dc.format.extent7 pages
dc.language.isoen_ZAen_ZA
dc.publisherBMC (part of Springer Nature)en_ZA
dc.subjectBovine tuberculosisen_ZA
dc.subjectWarthogs (Phacochoerus africanus) -- Diseasesen_ZA
dc.subjectTuberculin test -- South Africaen_ZA
dc.subjectAntigenic determinantsen_ZA
dc.titleMeasuring antigen-specific responses in Mycobacterium bovis-infected warthogs (Phacochoerus africanus) using the intradermal tuberculin testen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's versionen_ZA
dc.rights.holderAuthors retain copyrighten_ZA


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