ITEM VIEW

Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

dc.contributor.authorLiu, Zhuoen_ZA
dc.contributor.authorHo, Shih-Hsinen_ZA
dc.contributor.authorSasaki, Kengoen_ZA
dc.contributor.authorDen Haan, Riaanen_ZA
dc.contributor.authorInokuma, Kentaroen_ZA
dc.contributor.authorOgino, Chiakien_ZA
dc.contributor.authorVan Zyl, Willem H.en_ZA
dc.contributor.authorHasunuma, Tomohisaen_ZA
dc.contributor.authorKondo, Akihikoen_ZA
dc.date.accessioned2017-10-20T08:56:19Z
dc.date.available2017-10-20T08:56:19Z
dc.date.issued2016
dc.identifier.citationLiu, Z., et al. 2016. Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production. Scientific Reports, 6:24550, doi:10.1038/srep24550en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/srep24550
dc.identifier.urihttp://hdl.handle.net/10019.1/102361
dc.descriptionCITATION: Liu, Z., et al. 2016. Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production. Scientific Reports, 6:24550, doi:10.1038/srep24550.en_ZA
dc.descriptionThe original publication is available at http://www.nature.com/srepen_ZA
dc.description.abstractCellulosic biofuel is the subject of increasing attention. The main obstacle toward its economic feasibility is the recalcitrance of lignocellulose requiring large amount of enzyme to break. Several engineered yeast strains have been developed with cellulolytic activities to reduce the need for enzyme addition, but exhibiting limited effect. Here, we report the successful engineering of a cellulose-adherent Saccharomyces cerevisiae displaying four different synergistic cellulases on the cell surface. The cellulase-displaying yeast strain exhibited clear cell-to-cellulose adhesion and a “tearing” cellulose degradation pattern; the adhesion ability correlated with enhanced surface area and roughness of the target cellulose fibers, resulting in higher hydrolysis efficiency. The engineered yeast directly produced ethanol from rice straw despite a more than 40% decrease in the required enzyme dosage for high-density fermentation. Thus, improved cell-to-cellulose interactions provided a novel strategy for increasing cellulose hydrolysis, suggesting a mechanism for promoting the feasibility of cellulosic biofuel production.en_ZA
dc.description.urihttps://www.nature.com/articles/srep24550
dc.format.extent10 pages : illustrationsen_ZA
dc.language.isoen_ZAen_ZA
dc.publisherSpringer Natureen_ZA
dc.subjectCellulosic biofuel productionen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectCellulose biotechnologyen_ZA
dc.subjectCellulolytic activitiesen_ZA
dc.titleEngineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel productionen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's versionen_ZA
dc.rights.holderAuthors retain copyrighten_ZA


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

ITEM VIEW