The effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines
Date
1991
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries extracted from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV) and grapevine leafroll associated viruses (GLR) respectively. Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) as well as serological tests (ELISA). Results indicated that somatic embryogenesis derived from ovary tissue of infected grapevines is an effective technique to eliminate grapevine Ieafroll associated viruses from grapevines but the procedure was not successful in the elimination of GFL V from anther source material.
Description
CITATION: Goussard, P. G., Wiid, J. & Kasdor, G. G. F. 1991. The effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevine. South African Journal of Enology & Viticulture, 12(2):77-81, doi:10.21548/12-2-2214.
The original publication is available at http://www.journals.ac.za/index.php/sajev
The original publication is available at http://www.journals.ac.za/index.php/sajev
Keywords
Grapevine fanleaf virus, Grapevines -- Diseases and pests -- Control, Regeneration of somatic embryos
Citation
Goussard, P. G., Wiid, J. & Kasdor, G. G. F. 1991. The effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevine. South African Journal of Enology & Viticulture, 12(2):77-81, doi:10.21548/12-2-2214