Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity
Date
2006
Authors
Warren R.
De Kock M.
Engelke E.
Myburgh R.
Van Pittius N.G.
Victor T.
Van Helden P.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Description
Keywords
bacterial DNA, article, bactericidal activity, bacterium culture, DNA extraction, DNA fingerprinting, DNA isolation, heat, infection control, molecular epidemiology, Mycobacterium tuberculosis, nonhuman, priority journal, Bacteriological Techniques, DNA, Bacterial, Heat, Humans, Laboratory Infection, Mycobacterium Infections, Mycobacterium tuberculosis, Safety Management, Bacteria (microorganisms), Corynebacterineae, Mycobacterium tuberculosis
Citation
Journal of Clinical Microbiology
44
1
44
1