Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation
Date
2015-07-18
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Background: Neuroinflammation is central to the aetiology of HIV-associated neurocognitive disorders (HAND) that
are prevalent in late stage AIDS. Anti-retroviral (ARV) treatments are rolled out relatively late in the context of
neuroinflammatory changes, so that their usefulness in directly preventing HAND is probably limited. It is common
practice for HIV+ individuals in developing countries to make use of traditional medicines. One such medicine is
Sutherlandia frutescens - commonly consumed as a water infusion. Here its efficacy as an anti-inflammatory modality
in this context was investigated in an in vitro co-culture model of the blood–brain barrier (BBB).
Methods: Single cultures of human astrocytes (HA), HUVECs and primary human monocytes, as well as co-cultures
(BBB), were stimulated with HIV-1 subtype B & C Tat protein and/or HL2/3 cell secretory proteins after pre-treatment
with S.frutescens extract. Effects of this pre-treatment on pro-inflammatory cytokine secretion and monocyte migration
across the BBB were assessed.
Results: In accordance with others, B Tat was more pro-inflammatory than C Tat, validating our model. S.frutescens
decreased IL-1β secretion significantly (P < 0.0001), but exacerbated both monocyte chemoattractant protein-1
(P < 0001) – a major role player in HIV-associated neuroinflammation – and CD14+ monocyte infiltration across
the BBB (P < 0.01).
Conclusions: Current data illustrates that the combined use of HL2/3 cells and the simulated BBB presents an
accurate, physiologically relevant in vitro model with which to study neuroinflammation in the context of HIV/AIDS. In
addition, our results caution against the use of S.frutescens as anti-inflammatory modality at any stage post-HIV infection.
Description
CITATION: Africa, L. D. & Smith, C. 2015. Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation. Journal of Negative Results in BioMedicine., 14:14, doi:10.1186/s12952-015-0031-y.
The original publication is available at https://jnrbm.biomedcentral.com
The original publication is available at https://jnrbm.biomedcentral.com
Keywords
HIV infections, Inflammation, Sutherlandia frutescens, Blood-brain barrier
Citation
Africa, L. D. & Smith, C. 2015. Sutherlandia frutescens may exacerbate HIV-associated neuroinflammation. Journal of Negative Results in BioMedicine., 14:14, doi:10.1186/s12952-015-0031-y