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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Claassen-Weitz, Shantelle; Gardner-Lubbe, Sugnet; Mwaikono, Kilaza S.; Du Toit, Elloise; Zar, Heather J.; Nicol, Mark P. (BMC (part of Springer Nature), 2020-05-12)

Background: Careful consideration of experimental artefacts is required in order to successfully apply highthroughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, ...