Browsing by Author "Steytler J.G."

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    Agarose gel electrophoresis of glucose-6-phosphate-dehydrogenase isoenzymes
    (1991) Forbes J.; Steytler J.G.; Van Heerden R.
    [No abstract available]
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    Age and race distribution of patients who undergo haematological investigations at Tygerberg Hospital (Afrikaans)
    (HMPG, 1977-11) Brink S.; Van Schalkwyk D.J.; Steytler J.G.
    The race, age and sex of patients referred for peripheral blood investigations at the Tygerberg Hospital were analysed statistically. An IBM 370/158 computer was used. Two groups were compared. The first was a 'low Hb group' of 2065 patients who had at least once during the first 6 months of 1976 had a haemoglobin (Hb) value of 10 g/dl or lower. The second, a 'hospital group' of 600 patients, was selected with the aid of tables for generating random numbers and was representative of the general hospital population. Rank order correlation methods using the ratios between the observed and the population percentages (based on the census figures for the Cape Peninsula) for the different age groups, between the race-age combinations, were used, and significant differences were found. In the hospital group the higher percentage of women between 20 and 39 years and the higher percentage of men older than 60 years was striking. In the low Hb group 38.8% of the patients were below 4 years of age, and 61% in the microcytic group (mean corpuscular volume (MCV) below 75 fl) were under the age of 6 years. The largest number of cases of iron-deficient anaemias in the hospital was therefore found in children. The MCV and Hb values had a fairly normal distribution, but in the macrocytic subdivision (MCV higher than 95 fl) of the low Hb group, White men were significantly over-represented. In the hospital group Hb and MCV values were slightly lower than the values accepted in a normal population, the mean MCV of women being lower than that of men (P < 0.01), and the values of non-Whites being lower than those of Whites.
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    Apoptotic megakaryocyte dysplasia in the myelodysplastic syndromes
    (1992) Hatfill S.J.; Fester E.D.; Steytler J.G.
    The terminal phase of the megakaryocyte life span is characterized by the onset of apoptosis to form compact, denuded megakaryocyte nuclei (DMK) surrounded by a thin rim of cytoplasm. Increased numbers of DMK have been reported in patients with acquired immune deficiency syndrome (AIDS) and chronic myeloproliferative disorders. In this study the bone marrow biopsies of 20 patients with various FAB subtypes of myelodysplastic syndrome (MDS) were examined for the presence of DMK cells and semiquantified for marrow reticulin level. For all MDS subtypes, a 9% or greater incidence of DMK in the total megakaryocyte population of the bone marrow was associated with a significant deposit of reticulin in the marrow. Immunocytochemical staining for Factor VIII (Von Willebrand factor), showed the abnormal deposition of this megakaryocyte protein in the extravascular stroma around many of the DMK cells. These findings are consistent with a hypothesis for excess stromal reticulin based on the defective maturation and intramedullary death of large numbers of megakaryocytes. The number of DMK in the marrow biopsies of MDS patients may have prognostic significance.
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    Clinical and haematological problems associated with severe abruptio placentae
    (Health & Medical Publishing Group, 1978) Odendaal H.J.; Brink S.; Steytler J.G.
    A clinical study was done of 80 patients in whom abruptio placentae was severe enough to cause intra-uterine death of the fetus. Central venous pressure was measured whenever possible to ensure adequate fluid replacement. Although the minority of the patients were shocked when admitted, blood replacement averaged 5 units per patient. The caesarean section incidence was 21%. Only 1 patient developed renal failure for which peritoneal dialysis was done. No maternal deaths occurred. Serial coagulation studies were done in 58 patients. Diffuse intravascular coagulation was present in the majority. After delivery of the fetus, however, tests became normal.
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    Detection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in South Africa
    (1997) Engelbrecht S.; Treurnicht F.K.; Schneider J.W.; Jordaan H.F.; Steytler J.G.; Wranz P.A.B.; Van Rensburg E.J.
    The aetiology and detection of human herpes virus type 8 (HHV-8) DNA sequences in Kaposi's sarcoma (KS) is a matter of intense investigation. We report on the detection of HHV-8 DNA and sequence polymorphism in different clinicopathological subtypes of cutaneous KS samples from South Africa. The diagnosis was confirmed by histological examination in all cases. Six patients had classic KS (CKS), 3 epidemic KS (EKS), and 3 iatrogenic KS (IKS). A nested polymerase chain reaction (PCR) assay was used to detect HHV-8 DNA in cell lysates, prepared from formalin fixed, paraffin embedded sections. We investigated polymorphism in the HHV-8 DNA using single-stranded conformational polymorphism (SSCP) analysis on the PCR products, followed by direct sequencing. HHV-8 DNA was detected in all the patients with KS, irrespective of the clinicopathological subtype. Direct sequencing was performed on 5 selected cases and showed single base pair substitutions in all. The spectrum of mutations was similar to those described previously. No correlation was found between the different types of KS and sequence variation. The results support the hypothesis that HHV-8 is strongly associated with different clinicopathological subtypes of KS and confirm the occurrence of HHV-8 in patients with CKS, EKS, and IKS in South Africa.