Browsing by Author "Opara, Umezuruike Linus"

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    Advances in design and performance evaluation of fresh fruit ventilated distribution packaging : a review
    (Publishers version, 2020-02-17) Mukama, Matia; Ambaw, Alemayehu; Opara, Umezuruike Linus
    This review was initiated to realise the state-of-the art in optimising the ventilation and structural requirements of corrugated packaging carton design. Researchers have been using computational methods: computational fluid dynamics, particularly, the finite volume method, to analyse the airflow and heat transfer performances, and computational structural dynamics, particularly, the finite element method, to analyse the loss of compression strength due to vent-holes. Models are validated using actual testing: wind tunnel based forced air cooling system to study the produce cooling kinetics and box compression test machine for the package industry to study the structural dynamics. Studies on the rate and uniformity of produce cooling and the loss of structural strength in corrugated cartons as a function of size, shape, and location of vent-holes are reviewed. Based on experimental data, results show that the loss in strength can range between 10–40 % on addition of vent and hand holes on cartons, and reasonable increase in cooling rates is only achieved with increase in carton face ventilation area only up to 7–8 %. With regards to internal packaging components, increasing awareness of consumers to the environmental degradation of especially disposable plastic packaging means packers and suppliers must devise means to cut back and eventually eliminate plastic packaging from fruit and vegetables.
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    Application of dynamic controlled atmosphere technologies to reduce incidence of physiological disorders and maintain quality of 'Granny Smith' apples
    (MDPI, 2021-05-26) Kawhena, Tatenda Gift; Fawole, Olaniyi Amos; Opara, Umezuruike Linus
    The efficacy of dynamic controlled atmosphere technologies; repeated low oxygen stress (RLOS) and dynamic controlled atmosphere-chlorophyll fluorescence (DCA-CF) to control superficial scald development on ‘Granny Smith’ apples during long-term storage was studied. Fruit were stored for 2, 4, 6, 8, and 10 months at 0 °C in DCA-CF (0.6% O2 and 0.8% CO2), regular atmosphere (RA)(≈21% O2 and 90–95% RH), and RLOS treatments: (1) 0.5% O2 for 10 d followed by ultra-low oxygen (ULO) (0.9% O2 and 0.8% CO2) for 21 d and 0.5% O2 for 7 d or (2) 0.5% O2 for 10 d followed by controlled atmosphere (CA) (1.5% O2 and 1% CO2) for 21 d and 0.5% O2 for 7 d. Development of superficial scald was inhibited for up to 10 months and 7 d shelf life (20 °C) under RLOS + ULO and DCA-CF treatments. Apples stored in RLOS + ULO, RLOS + CA, and DCA-CF had significantly (p < 0.05) higher flesh firmness and total soluble solids. The RLOS phases applied with CA or ULO and DCA-CF storage reduced the development of superficial scald by possibly suppressing the oxidation of volatiles implicated in superficial scald development.
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    Application of gum arabic and methyl cellulose coatings enriched with thyme oil to maintain quality and extend shelf life of “Acco” pomegranate arils
    (MDPI, 2020-12-01) Kawhena, Tatenda Gift; Tsige, Alemayehu Ambaw; Opara, Umezuruike Linus; Fawole, Olaniyi Amos
    The effects of gum arabic (GA; 1.5% w/v) and methyl cellulose (MC; 1% w/v) enriched with thyme oil (TO; 0.25 and 0.5% v/v) on the quality of “Acco” pomegranate arils were studied. Coating treatments, namely, MC, MC + TO0.5%, MC + TO0.25%, GA, GA + TO0.5% and GA + TO0.25% were applied on arils by dipping, and evaluations were made on physicochemical and microbiological quality, phytochemicals and antioxidant capacity of arils stored (5 ± 1 ◦C, 95 ± 2% RH) for 16 days. Dipping arils in GA or MC, both containing TO (0.5% v/v) significantly (p < 0.0001) reduced weight loss and enhanced antioxidant activity (FRAP) (p = 0.0014). However, dipping arils in GA combined with TO (0.25% v/v) had the highest influence on reducing aril respiration rate compared with other treatments. Overall, results showed that application of coatings (GA + TO0.5% and GA + TO0.25%) reduced total yeast and mould and total plate counts and maintained quality up to 8 days of storage. These findings suggest that either GA + TO0.5% or GA + TO0.25% coatings have the capability to extend storage life of “Acco” pomegranate arils.
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    Building human capacity for our future.
    (South African Fruit Journal, 2009) Opara, Umezuruike Linus
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    CFD model development and validation of a thermonebulisation fungicide fogging system for postharvest storage of fruit
    (Elsevier, 2012-01) Delele, M. A.; Vorstermans, B.; Creemers, P.; Tsige, A. A.; Tijskens, E.; Schenk, A.; Opara, Umezuruike Linus; Nicolai, B. M.; Verboven, P.
    Postharvest treatments of fruits in storage rooms using a fogging system is a recent development that offer a promising means to reduce the use of fungicides in the orchards, where ecological and environmental risks are higher than in the closed environment of the storage room. To investigate the effectiveness of postharvest storage fungicide fogging systems, a computational fluid dynamics (CFD) model was developed and validated. A discrete element (DE) method was applied to generate a random stacking of spherical fruits in a typical bin. The CFD model was then employed to study explicitly the air and fungicide particle flow through the bin vent holes and through the voids of the stack, and to predict the deposition behaviour of the fungicide particle on the products. For model validation purposes, a standardized set-up was used with a single fruit bin positioned in a cold store that was operated at different air flow rates. Good agreement was found between measured and predicted results of deposition profiles of fungicide particles. The deposition on the top layer of the fruit stack was higher than the bottom one, and higher deposition was observed on the top sides than on the bottom sides of the fruit. The effect of air flow rates and different bin handling parameters on fungicide particles flow and deposition were investigated. Air circulation rates of 0 m3 h 1 (no air circulation), 4080 m3 h 1 and 6800 m3 h 1 and 9520 m3 h 1 were used. The highest fungicide deposition on the fruit was observed during fogging without air circulation while the lowest deposition corresponded to fogging with the highest air circulation rate. Covering the top of the bin with foil and removing the bottom plastic foam that is usually placed on the bottom floor of the bin improves the uniformity of fungicide deposition throughout the bin. Removing the bottom plastic liner increased the average deposition of the fungicide particles, while covering the top of the bin decreased the average deposition
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    Challenging our postharvest technology research and innovation system.
    (South African Fruit Journal, 2009) Opara, Umezuruike Linus
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    Changes in physical properties, chemical and elemental composition and antioxidant capacity of pomegranate (cv. Ruby) fruit as five maturity stages
    (Elsevier, 2013) Fawole, Olaniyi A.; Opara, Umezuruike Linus
    This study was conducted to investigate the physical and compositional changes as well as antioxidant properties of pomegranate fruit (cv. Ruby) at five distinct maturity stages between 54 and 139 days after full bloom (DAFB). Physico-chemical properties such as fruit mass, size, juiciness, colour, total soluble solids (TSS), pH, titratable acidity (TA), individual organic acids and sugars, and phenolic composition were investigated. Mineral element concentrations were determined using inductively coupled plasma optical emission spectrometry (ICP-OES) while total antioxidant capacity was measured by DPPH scavenging activity (DPPH) and ferric ion reducing power (FRAP). Results showed that major compositional changes in fruit are developmentally regulated. Significant increases in total soluble solids (TSS), sugars (glucose and fructose) and anthocyanins composition, coupled with significant decline in titratable acidity (TA), organic acids and total phenolics occurred with advancing maturity. Principal component analysis (PCA) showed that fruit at advanced maturity stages (132 and 139 DAFB) were characterized by intense peel and aril pigmentation and better juice quality. PCA results also showed that peel and aril colour attributes and indices of sugar/acid ratio (TSS/TA and BrimA index) could be useful measures of fruit maturity and ripeness for ‘Ruby’ pomegranate cultivar, and therefore might be suitable for the development of reliable fruit maturity index to assess fruit optimal maturity.
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    Chemical and phytochemical properties and antioxidant activities of three pomegranate cultivars grown in South Africa
    (Springer Verlag, 2011-02) Fawole, Olaniyi A.; Opara, Umezuruike Linus; Theron, K. I.
    A comparative study of chemical contents and antioxidant activities of three pomegranate cultivars (‘Arakta’, ‘Bhagwa’ and ‘Ruby’) grown in South Africa was conducted. Fresh pomegranate juice (PJ) of each cultivar were assessed for soluble solid contents (SSCs), pH and titratable acidity (TA), while extracted juice samples were evaluated for total phenolic (TP), including total tannins (TT), proanthocyanidins (Pcy), total flavonoids, anthocyanins and gallic acids (GA) using spectrophotometric methods. The antioxidant properties of the juice samples were evaluated against stable 2, 2– diphenyl–1–picryl hydrazyl, as well as in ferric reducing ability of plasma (FRAP) and QuantiChrom™ (TAC) antioxidant assays. There were significant differences in the chemical properties of the cultivars. SSC, TA and pH varied between the range of 14.07–15.10 °Brix, 0.22– 0.28 g/100 ml and 3.32–3.64, respectively. ‘Bhagwa’ had the highest TP (449.9 mg/100 ml), 1.3-fold and 1.6-fold higher than ‘Arakta’ and ‘Ruby’, respectively. The strongest total antioxidant activity was exhibited by ‘Bhagwa’ with an antioxidant index of 95.7%, followed by ‘Arakta’ (93.2%) and ‘Ruby’ (79.9%). PJ phytochemical properties (TP, TT, Pcy, GA) and antioxidant activity (FRAP and TAC) were significantly correlated (r2= 0.509–0.885) with each other.
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    Classification learning of latent bruise damage to apples using shortwave infrared hyperspectral imaging
    (MDPI, 2021-07-22) Nturambirwe, Jean Frederic Isingizwe; Perold, Willem Jacobus; Opara, Umezuruike Linus
    Bruise damage is a very commonly occurring defect in apple fruit which facilitates disease occurrence and spread, leads to fruit deterioration and can greatly contribute to postharvest loss. The detection of bruises at their earliest stage of development can be advantageous for screening purposes. An experiment to induce soft bruises in Golden Delicious apples was conducted by applying impact energy at different levels, which allowed to investigate the detectability of bruises at their latent stage. The existence of bruises that were rather invisible to the naked eye and to a digital camera was proven by reconstruction of hyperspectral images of bruised apples, based on effective wavelengths and data dimensionality reduced hyperspectrograms. Machine learning classifiers, namely ensemble subspace discriminant (ESD), k-nearest neighbors (KNN), support vector machine (SVM) and linear discriminant analysis (LDA) were used to build models for detecting bruises at their latent stage, to study the influence of time after bruise occurrence on detection performance and to model quantitative aspects of bruises (severity), spanning from latent to visible bruises. Over all classifiers, detection models had a higher performance than quantitative ones. Given its highest speed in prediction and high classification performance, SVM was rated most recommendable for detection tasks. However, ESD models had the highest classification accuracy in quantitative (>85%) models and were found to be relatively better suited for such a multiple category classification problem than the rest.
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    Classification of 'Granny Smith' apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis
    (German Botanical Society, 2016) Mditshwa, Asanda; Fawole, Olaniyi A.; Vries, Filicity; Van der Merwe, Kobus; Crouch, Elke; Opara, Umezuruike Linus
    To study the metabolic changes in ‘Granny Smith’ apples with different severities of superficial scald, fruit were stored in normal refrigerated air (0°C, 95% RH) for 12 weeks followed by 7 d shelf-life under room conditions (20°C, 65% RH). Fruit were graded to five groups based on scald severity and analysed for ethylene, α-farnesene and 6-methyl-5-hepten-2-one (MHO) levels. Reactive oxygen species (ROS) were measured by confocal laser-scanning microscopy on apple peel treated with fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate. Ethylene production rate, α-farnesene and MHO contents and ROS intensity increased with increasing scald severity but declined in severely scalded fruit. Malondialdehyde (MDA) concentration in fruit peel, a measure of membrane damage, increased linearly (R=0.891) with increase in scald severity. Discriminant analysis was used to classify fruit by scald severity on the basis of metabolites accumulated. The stepwise model indicated that three attributes (ROS, ethylene production and MDA) contributed significantly (R2≥0.5) to the separation of the five scald severity indexes, with ROS having the highest contribution (partial R² =0.961; p<0.0001), followed by ethylene (R2=0.718; p<0.0001) and MDA (R2=0.578; p<0.0001).
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    Comparative study of phytochemical contents and antioxidant properties of juice of three pomegranate fruit cultivars grown in South Africa
    (2011) Fawole O.A.; Opara, Umezuruike Linus; Theron K.I.
    [No abstract available]
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    Design of Packaging Vents for Cooling Fresh Horticultural Produce
    (2012) Pathare P.B.; Opara, Umezuruike Linus; Vigneault C.; Delele M.A.; Al-Said F.A.-J.
    This review focuses on the design of vents in packages used for handling horticulture produce. The studies on vent designs that are conducted to obtain fundamental understanding of the mechanisms by which different parameters affect the rate and homogeneity of the airflow and the cooling process are presented. Ventilated packages should be designed in such a way that they can provide a uniform airflow distribution and consequently uniform produce cooling. Total opening area and opening size and position show a significant effect on pressure drop, air distribution uniformity and cooling efficiency. Recent advances in measurement and mathematical modelling techniques have provided powerful tools to develop detailed investigations of local airflow rate and heat and mass transfer processes within complex packaging structures. The complexity of the physical structure of the packed systems and the biological variability of the produce make both experimental and model-based studies of transport processes challenging. In many of the available mathematical models, the packed structure is assumed as a porous medium; the limitations of the porous media approach are evident during vented package design studies principally when the container-to-produce dimension ratio is below a certain value. The complex and chaotic structure within horticultural produce ventilated packages during a forced-air precooling process complicates the numerical study of energy and mass transfer considering each individual produce. Future research efforts should be directed to detailed models of the vented package, the complex produce stacking within the package, as well as their interaction with adjacent produce, stacks and surrounding environment. For the validation of the numerical models, the development of better experimental techniques taking into account the complex packaging system is also very important. © 2012 Springer Science+Business Media, LLC.
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    Drying kinetics of pomegranate fruit peel (cv. Wonderful)
    (Elsevier, 2019) Mphahlele, Rebogile R.; Pathare, Pankaj B.; Opara, Umezuruike Linus
    Pomegranate juice processing produces large amount of peel as by-product or waste which is a potential source of raw material for food and other bioprocessing industries. Drying the peel offers opportunities for value addition into novel products, thus reducing waste from the fruit processing operations. This study presents the mathematical models of thin layer drying behaviour of pomegranate peels (initial thickness 5.00 ± 0.05 mm and moisture content 70.30% wet basis) using three air temperatures (40 °C, 50 °C and 60 °C) at a constant air velocity of 1.0 m/s. The results obtained showed that drying time decreased as the oven drying temperature increased. The drying process took place mainly in the falling rate period. Ten thin layer drying models were evaluated based on coefficient of determination (r2) and standard error (es). Among the tested drying models, Midilli et al. mathematical model was found to be the best fit for establishing the drying kinetics of pomegranate peel. Furthermore, the effective moisture diffusivity of pomegranate peel ranged from 4.05 × 10−10 to 8.10 × 10−10 m2/s over the temperature range investigated, with mean activation energy (Ea) of 22.25 kJ/mol.
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    Effect of blanching pomegranate seeds on physicochemical attributes, bioactive compounds and antioxidant activity of extracted oil
    (MDPI, 2020-05-31) Kaseke, Tafadzwa; Opara, Umezuruike Linus; Fawole, Olaniyi Amos
    This study investigated the effect of blanching pomegranate seeds (PS) on oil yield, refractive index (RI), yellowness index (YI), conjugated dienes (K232), conjugated trienes (K270), total carotenoid content (TCC), total phenolic compounds (TPC) and DPPH radical scavenging of the extracted oil. Furthermore, phytosterol and fatty acid compositions of the oil extracted under optimum blanching conditions were compared with those from the oil extracted from unblanched PS. Three different blanching temperature levels (80, 90, and 100 °C) were studied at a constant blanching time of 3 min. The blanching time was then increased to 5 min at the established optimum blanching temperature (90 °C). Blanching PS increased oil yield, K232, K270, stigmasterol, punicic acid, TPC and DPPH radical scavenging, whereas YI, β-sitosterol, palmitic acid and linoleic acid were decreased. The RI, TCC, brassicasterol, stearic acid, oleic acid and arachidic acid of the extracted oil were not significantly (p > 0.05) affected by blanching. Blanching PS at 90 °C for 3 to 5 min was associated with oil yield, TPC and DPPH. Blanching PS at 90 °C for 3 to 5 min will not only increase oil yield but could also improve functional properties such as antioxidant activity, which are desirable in the cosmetic, pharmaceutical, nutraceutical and food industries.
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    Effect of hot-air and freeze-drying on the quality attributes of dried pomegranate (Punica granatum L.) arils during long-term cold storage of whole fruit
    (MDPI, 2020-10-22) Adetoro, Adegoke Olusesan; Opara, Umezuruike Linus; Fawole, Olaniyi Amos
    : This study investigated the effect of hot-air and freeze-drying on the physicochemical, phytochemical and antioxidant capacity of dried pomegranate arils during long-term cold storage (7 ± 0.3 ◦C, with 92 ± 3% relative humidity) of whole fruit over a single experiment. Extracted arils were processed at monthly intervals during 12 weeks of cold storage of whole fruit. After the 12-week storage period, hot-air and freeze-dried arils showed the least (3.02) and highest (23.6) total colour difference (TCD), respectively. Hot-air dried arils also contained 46% more total soluble solids (TSS) than freeze-dried arils. During the storage of pomegranate fruit, total phenolic content (TPC) steadily increased from 20.9 to 23.9 mg GAE/100 mL and total anthocyanin content (TAC) increased from 6.91 to 8.77 mg C3gE /100 mL. Similarly, an increase in TPC and TAC were observed for hot-air (9.3%; 13%) and freeze-dried arils (5%; 5%), respectively. However, the radical scavenging activity (RSA) reduced by 8.5 and 17.4% for hot-air and freeze-dried arils, respectively, after 12 weeks of cold storage. Overall, the parameters such as colour, TPC and TAC as well as the lower degradation in RSA stability during storage showed distinct differences in quality when using the freeze-drying method, which is, therefore, recommended.
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    Effects of cooking methods on carotenoids content of Kingfish.
    (British Food Journal, 2010) Opara, Umezuruike Linus; Al-Ani MR
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    Effects of packaging liners on cooling rate and quality attributes of table grape (cv. Regal Seedless)
    (Wiley Blackwell, 2011-07) Ngcobo, Mduduzi E. K.; Opara, Umezuruike Linus; Thiart, G. D.
    Table grapes are commonly packed in multi‐layered packages consisting of cardboard carton, plastic liners and carry bag/pouch to maintain product quality along the cold chain. Each liner is characterized by the number and the size of perforations, which influence the environmental conditions around the produce inside the package. This study investigated the effects of different carton liners on the cooling rate and quality attributes of ‘Regal Seedless’ table grapes. Fruit quality attributes measured include weight loss, stem dehydration and browning, SO2 injury, decay, berry firmness and colour. Non‐perforated liners maintained relative humidity (RH) close to 100% during cold storage and during a 7 day shelf life period, which resulted in delaying the loss of stem quality but significantly (p≤0.05) increasing the incidence of SO2 injury and berry drop during storage, as compared with perforated liners. Perforated liners improved fruit cooling rates but significantly (p≤0.05) reduced RH. Low RH in perforated liners resulted in significant (p≤0.05) increase in stem dehydration and browning as compared with non‐perforated liners. Berry firmness decreased by 78% after 42 days of storage at −0.5°C. Significant (p≤0.05) differences in berry firmness between the different packages were observed only during the first 7 days of storage. Berry colour changed from greenish‐yellow to yellowish‐green in all types of plastic liner during the storage period. Fruit decay occurred in all packages after a 7 day shelf life, with the highest incidence of decay occurring in liners with less perforation