Cardiothoracic Surgery
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Browsing Cardiothoracic Surgery by Author "Bester, A. J."
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- Item'n Nuwe metode vir die bepaliog van serotonien in bloed en serebrospinale vloeistof(Health & Medical Publishing Group, 1979) Kriek, J. A.; Bester, A. J.; Rossouw, J. J.A new rapid method for the microdetermination of serotonin (5-hydroxytryptamine) in plasma and cerebrospinal fluid is described. A recovery coefficient of 98% was obtained in the present method, with the use of [3H]-hydroxytryptamine creatinine sulphate as an internal standard, compared with recovery coefficients of 31% and 66% for the two existing methods generally used for serotonin determination. This method involves a one-step sample deproteinization by perchloric acid, resulting in a high stability of serotonin.