Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet

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dc.contributor.authorO'Kennedy M.M.
dc.contributor.authorCrampton B.G.
dc.contributor.authorLorito M.
dc.contributor.authorChakauya E.
dc.contributor.authorBreese W.A.
dc.contributor.authorBurger J.T.
dc.contributor.authorBotha F.C.
dc.date.accessioned2011-05-25T08:49:47Z
dc.date.available2011-05-25T08:49:47Z
dc.date.issued2011
dc.description.abstractSclerospora graminicola is an oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this cereal crop. In this study a hydrolytic enzyme, β-1,3-glucanase (gluc78), from the biocontrol fungus Trichoderma atroviride, was introduced into the genome of a pearl millet breeding line, 842B, by particle bombardment. Constructs were prepared containing the gluc78 gene, encoding the 78. kDa β-1,3-glucanase protein, downstream of either the constitutive ubiquitin promoter or the wound inducible potato proteinase inhibitor IIK gene promoter (pin2). The positive selectable marker gene, manA, encoding mannose-6-phosphate isomerase (phosphomannose isomerase) under the control of the ubiquitin promoter, was used for co-transformation. Transgenic plants were obtained harbouring the manA selectable marker gene and the antifungal gene gluc78 downstream of either the ubiquitin or pin2 promoter. Full constructs or minimal transgene expression cassettes containing the genes of interest were successfully introduced into the genome of pearl millet. Progeny of stably transformed plants, harbouring the gluc78 transgene which is driven by the pin2 promoter and followed by the rice Act1 intron sequences, was subjected to pathogenicity trials. One transgenic event exhibited a reduction of 58% in the incidence of S. graminicola infection, however other transgenic pearl millet events showed no resistance to this phytopathogen. The event conferring decreased susceptibility to S. graminicola had high levels of the glucanase transcript especially in transgenic plants showing higher levels of downy mildew infection. © 2010 SAAB.
dc.description.versionArticle
dc.identifier.citationSouth African Journal of Botany
dc.identifier.citation77
dc.identifier.citation2
dc.identifier.citation335
dc.identifier.citation345
dc.identifier.issn2546299
dc.identifier.other10.1016/j.sajb.2010.09.016
dc.identifier.urihttp://hdl.handle.net/10019.1/14691
dc.titleExpression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet
dc.typeArticle
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