Genetic diversity of Mycobacterium tuberculosis complex strains isolated from livestock workers and cattle in Nigeria
Date
2019-02-20
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS
Abstract
ENGLISH ABSTRACT: Molecular typing techniques are useful in understanding tuberculosis epidemiology; yet,
they have been under-utilised at the human-animal interface in Nigeria. Sixty-four Mycobacterium tuberculosis complex (MTBC) isolates including 42 M. tuberculosis, 13 M. bovis and
nine M. africanum obtained from livestock workers (LW, n = 47) and their cattle (n = 17) in
three geographical zones of Nigeria were genotyped to identify and evaluate the genetic
diversity of the circulating MTBC using spoligotyping. Distribution into clades of M. tuberculosis revealed; 45.3% Uganda I- [SIT46- cattle: 1; LW: 28], 14.1% Latin American Mediterranean- [SIT61, cattle: 1; LW: 8], and 1.6% T- [SIT53—LW: 1]. The M. bovis strains were
6.3% SB0944 [cattle: 4] and 1.6% each of SB0300, SB1026, SB1027 and SB1439 [cattle:
4]. Seventeen MTBC isolates [cattle: 7; LW: 10] yielded 14 new spoligotype patterns including three M. tuberculosis strains (three isolates), five M. bovis strains (five isolates) and six
M. africanum strains (nine isolates), two of which belonged to MAF1. Only few families
namely, the not previously described Uganda I-, LAM and SB0944 are predominant among
the LW and cattle, with other types in lower prevalences. The strain population structure
indicates an intriguing diversity and possible zoonotic linkage with consequences for TB
control in the country. The need to employ newer molecular techniques such as Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats and whole genome
sequence to decipher circulating MTBC strains in Nigeria is advocated.
Description
CITATION: Adesokan, H. K. et al. 2019. Genetic diversity of Mycobacterium tuberculosis complex strains isolated from livestock workers and cattle in Nigeria. PLoS ONE, 14(2):e0211637, doi:10.1371/journal.pone.0211637.
The original publication is available at https://journals.plos.org/plosone
The original publication is available at https://journals.plos.org/plosone
Keywords
Mycobacterium tuberculosis, Livestock workers, Tuberculosis in cattle, Diseases -- Genetic aspects
Citation
Adesokan, H. K. et al. 2019. Genetic diversity of Mycobacterium tuberculosis complex strains isolated from livestock workers and cattle in Nigeria. PLoS ONE, 14(2):e0211637, doi:10.1371/journal.pone.0211637.