Ameliorative potentials of quercetin against cotinine-induced toxic effects on human spermatozoa

dc.contributor.authorGoss, Daleen_ZA
dc.contributor.authorOyeyipo, Ibukun P.en_ZA
dc.contributor.authorSkosana, Bongekile T.en_ZA
dc.contributor.authorAyad, Bashir M.en_ZA
dc.contributor.authorDu Plessis, Stefan S.en_ZA
dc.date.accessioned2017-07-06T13:05:49Z
dc.date.available2017-07-06T13:05:49Z
dc.date.issued2016
dc.descriptionCITATION: Goss, D., et al. 2016. Ameliorative potentials of quercetin against cotinine-induced toxic effects on human spermatozoa. Asian Pacific Journal of Reproduction, 5(3):193-197, doi:10.1016/j.apjr.2016.03.005.
dc.descriptionThe original publication is available at http://www.apjr.net/
dc.description.abstractObjectives: Cotinine, the principal metabolite of nicotine found in smokers' seminal plasma, has been shown to adversely affect sperm functionality while quercetin, a flavonoid with diverse properties is associated with several in vivo and in vitro health benefits. The aim of this study was to investigate the potential benefits of quercetin supplementation against damage caused by the by-products of tobacco smoke in human sperm cells. Methods: Washed human spermatozoa from 10 normozoospermic donors were treated with nutrient medium (control), quercetin (30 mmol/L) and cotinine (190 mg/mL, 300 ng/mL) with or without quercetin for 60 and 180 min incubation periods. Computer-aided sperm analysis was used to assess sperm motility while acrosomereacted cells were identified under a fluorescent microscope using fluorescein isothiocyanate-labelled Pisum Sativum Agglutinin as a probe, viability was assessed by means of a dye exclusion staining technique (eosin/nigrosin) and oxidative stress by flow cytometry using dihydroethidium as a probe. Values were expressed as mean ± S.E.M. as compared by ANOVA. Results: Higher cotinine concentrations reduced the number of viable cells after 60 and 180 min of exposure while viability of cells was increased in the cotinine aliquots supplemented with quercetin after 180 min of exposure when compared with cotinine only treated group. Conclusion: This study indicates that the ameliorating ability of quercetin on cotinineinduced decline in sperm function is associated with increased number of viable cells.en_ZA
dc.description.urihttp://www.sciencedirect.com/science/article/pii/S2305050016300045
dc.description.versionAuthors retain copyright
dc.format.extent5 pages
dc.identifier.citationGoss, D., et al. 2016. Ameliorative potentials of quercetin against cotinine-induced toxic effects on human spermatozoa. Asian Pacific Journal of Reproduction, 5(3):193-197, doi:10.1016/j.apjr.2016.03.005
dc.identifier.issn2305-0519 (online)
dc.identifier.issn2305-0500 (print)
dc.identifier.otherdoi:10.1016/j.apjr.2016.03.005
dc.identifier.urihttp://hdl.handle.net/10019.1/101928
dc.language.isoen_ZAen_ZA
dc.publisherHainan Medical University Journal Publisher
dc.rights.holderAsian Pacific Journal of Reproduction
dc.subjectCotinine -- Physiological effecten_ZA
dc.subjectQuercetin -- Health benefitsen_ZA
dc.subjectSpermatozoa -- Effect of smoking onen_ZA
dc.subjectSmoking -- Physiological effecten_ZA
dc.titleAmeliorative potentials of quercetin against cotinine-induced toxic effects on human spermatozoaen_ZA
dc.typeArticleen_ZA
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