Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model

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dc.contributor.authorPluddemann A.
dc.contributor.authorvan Zyl W.H.
dc.date.accessioned2011-05-15T16:01:28Z
dc.date.available2011-05-15T16:01:28Z
dc.date.issued2003
dc.description.abstractThe filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene. Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation43
dc.identifier.citation6
dc.identifier.issn1728083
dc.identifier.other10.1007/s00294-003-0409-0
dc.identifier.urihttp://hdl.handle.net/10019.1/11998
dc.subjectdimer
dc.subjectglyceraldehyde 3 phosphate dehydrogenase
dc.subjecthepatitis B surface antigen
dc.subjectrecombinant protein
dc.subjectvirus envelope protein
dc.subjectvitronectin
dc.subjectarticle
dc.subjectAspergillus nidulans
dc.subjectAspergillus niger
dc.subjectcost effectiveness analysis
dc.subjectelectron microscopy
dc.subjectfungal strain
dc.subjectfungus culture
dc.subjectgene cassette
dc.subjectgene expression
dc.subjectgenetic transcription
dc.subjectgenetic transformation
dc.subjectgenome
dc.subjectHepatitis B virus
dc.subjecthost
dc.subjectnonhuman
dc.subjectparticle size
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein purification
dc.subjectprotein synthesis
dc.subjectvaccine production
dc.subjectvirus gene
dc.subjectvirus particle
dc.subjectWestern blotting
dc.subjectAspergillus niger
dc.subjectBioreactors
dc.subjectBiotechnology
dc.subjectGene Dosage
dc.subjectGenetic Engineering
dc.subjectHepatitis B Surface Antigens
dc.subjectHepatitis B virus
dc.subjectHumans
dc.subjectModels, Genetic
dc.subjectPromoter Regions (Genetics)
dc.subjectTransformation, Genetic
dc.subjectTransgenes
dc.subjectViral Proteins
dc.subjectVirion
dc.subjectAspergillus
dc.subjectAspergillus niger
dc.subjectEmericella nidulans
dc.subjectFungi
dc.subjectHepatitis B virus
dc.titleEvaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model
dc.typeArticle
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