A new antigen scanning strategy for monitoring HIV-1 specific T-cell immune responses

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dc.contributor.authorMalnati M.S.
dc.contributor.authorHeltai S.
dc.contributor.authorCosma A.
dc.contributor.authorReitmeir P.
dc.contributor.authorAllgayer S.
dc.contributor.authorGlashoff R.H.
dc.contributor.authorLiebrich W.
dc.contributor.authorVardas E.
dc.contributor.authorImami N.
dc.contributor.authorWestrop S.
dc.contributor.authorNozza S.
dc.contributor.authorTambussi G.
dc.contributor.authorButto S.
dc.contributor.authorFanales-Belasio E.
dc.contributor.authorEnsoli B.
dc.contributor.authorEnsoli F.
dc.contributor.authorTripiciano A.
dc.contributor.authorFortis C.
dc.contributor.authorLusso P.
dc.contributor.authorPoli G.
dc.contributor.authorErfle V.
dc.contributor.authorHolmes H.
dc.date.accessioned2012-01-18T08:06:15Z
dc.date.available2012-01-18T08:06:15Z
dc.date.issued2012-01-18
dc.descriptionPlease help us populate SUNScholar with the post print version of this article. It can be e-mailed to: scholar@sun.ac.za
dc.description.abstractDelineation of the immune correlates of protection in natural infection or after vaccination is a mandatory step for vaccine development. Although the most recent techniques allow a sensitive and specific detection of the cellular immune response, a consensus on the best strategy to assess their magnitude and breadth is yet to be reached. Within the AIDS Vaccine Integrated Project (AVIP http://www.avip-eu.org) we developed an antigen scanning strategy combining the empirical-based approach of overlapping peptides with a vast array of database information. This new system, termed Variable Overlapping Peptide Scanning Design (VOPSD), was used for preparing two peptide sets encompassing the candidate HIV-1 vaccine antigens Tat and Nef. Validation of the VOPSD strategy was obtained by direct comparison with 15mer or 20mer peptide sets in a trial involving six laboratories of the AVIP consortium. Cross-reactive background responses were measured in 80 HIV seronegative donors (HIV-), while sensitivity and magnitude of Tat and Nef-specific T-cell responses were assessed on 90 HIV+ individuals. In HIV-, VOPSD peptides generated background responses comparable with those of the standard sets. In HIV-1+ individuals the VOPSD pools showed a higher sensitivity in detecting individual responses (Tat VOPSD vs. Tat 15mers or 20mers: p ≤ 0.01) as well as in generating stronger responses (Nef VOPSD vs. Nef 20mers: p < 0.001) than standard sets, enhancing both CD4 and CD8 T-cell responses. Moreover, this peptide design allowed a marked reduction of the peptides number, representing a powerful tool for investigating novel HIV-1 candidate vaccine antigens in cohorts of HIV-seronegative and seropositive individuals. © 2011 Elsevier B.V.
dc.identifier.citationJournal of Immunological Methods
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80053981846&partnerID=40&md5=0ecb26eecc7bfc78741d5516b140c8de
dc.identifier.issn221759
dc.identifier.other10.1016/j.jim.2011.09.005
dc.identifier.urihttp://hdl.handle.net/10019.1/18735
dc.titleA new antigen scanning strategy for monitoring HIV-1 specific T-cell immune responses
dc.typeArticle in Press
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