In vitro influence of oxygenation on enzymes of rat adipose tissue.

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dc.contributor.authorMacfarlane C.M.
dc.date.accessioned2011-05-15T16:16:07Z
dc.date.available2011-05-15T16:16:07Z
dc.date.issued1992
dc.description.abstractPre-incubation of rat adipose tissue from ad lib fed rats in the presence of 1.2 U/ml insulin and 2.8 x 10(-7) M prostaglandin F2 alpha for 16 hours in a gas atmosphere of 5% O2 led to an increase in glucose-6-phosphate dehydrogenase activity. No increase in glycerol-3-phosphate dehydrogenase or hexokinase activities were noted. Actinomycin D (50 micrograms/ml) and dehydroepiandrosterone (120 micrograms/ml) attenuated this increase and the increase in incorporation of [1-14C] glucose into carbon dioxide and tissue seen under these conditions. No increase in glucose-6-phosphate dehydrogenase was seen when pre-incubation was made in a gas atmosphere containing 20% O2. Although dehydroepiandrosterone inhibited both enzyme activity and isotope incorporation in a 20% O2 gas atmosphere, actinomycin D was without effect.
dc.description.versionArticle
dc.identifier.citationClinical Physiology and Biochemistry
dc.identifier.citation9
dc.identifier.citation2
dc.identifier.issn02521164
dc.identifier.urihttp://hdl.handle.net/10019.1/13643
dc.subjectdactinomycin
dc.subjectglucose 6 phosphate dehydrogenase
dc.subjectglycerol 3 phosphate dehydrogenase
dc.subjecthexokinase
dc.subjectinsulin
dc.subjectoxygen
dc.subjectprasterone
dc.subjectprostaglandin F2 alpha
dc.subjectadipose tissue
dc.subjectanimal
dc.subjectarticle
dc.subjectcell culture
dc.subjectdrug effect
dc.subjectenzymology
dc.subjectmetabolism
dc.subjectrat
dc.subjectAdipose Tissue
dc.subjectAnimal
dc.subjectCells, Cultured
dc.subjectDactinomycin
dc.subjectDinoprost
dc.subjectGlucosephosphate Dehydrogenase
dc.subjectGlycerolphosphate Dehydrogenase
dc.subjectHexokinase
dc.subjectInsulin
dc.subjectOxygen
dc.subjectPrasterone
dc.subjectRats
dc.subjectSupport, Non-U.S. Gov't
dc.titleIn vitro influence of oxygenation on enzymes of rat adipose tissue.
dc.typeArticle
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