A comparative study of the effects of cholesterol, beta-sitosterol, beta-sitosterol glucoside, dehydroepiandrosterone sulphate and melatonin on in vitro lipid peroxidation

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dc.contributor.authorVan Rensburg S.J.
dc.contributor.authorDaniels W.M.U.
dc.contributor.authorVan Zyl J.M.
dc.contributor.authorTaljaard J.J.F.
dc.date.accessioned2011-05-15T16:17:23Z
dc.date.available2011-05-15T16:17:23Z
dc.date.issued2000
dc.description.abstractThe free radical scavenging abilities of the structurally related steroids beta-sitosterol, beta-sitosterol glucoside (plant sterols and sterolins), cholesterol, and dehydroepiandrosterone sulphate (DHEAS) were compared with melatonin (an efficient free radical scavenger) in an in vitro system which measures lipid peroxidation of platelet membranes in the presence of iron (Fe2+). Lipid peroxidation is a process whereby cellular membranes are damaged due to the oxidative deterioration of polyunsaturated lipids, which may lead to cell death and disease in living organisms. Substances such as vitamin E protect cellular membranes against oxidative damage due to their chemical structures. The steroids cholesterol, beta-sitosterol, beta-sitosterol glucoside and dehydroepiandrosterone (DHEA) are structurally related to each other. During aging, serum concentrations of DHEA, DHEAS and melatonin decrease, while the concentration of cholesterol tends to increase. The aim of the present study was to compare the role these substances play in lipid peroxidation over a wide concentration range. At concentrations lower than the free iron in the reaction mixture, all the steroids investigated decreased lipid peroxidation. At higher concentrations, cholesterol and beta-sitosterol increased lipid peroxidation, while DHEAS and melatonin continued to decrease lipid peroxidation.
dc.description.versionConference Paper
dc.identifier.citationMetabolic Brain Disease
dc.identifier.citation15
dc.identifier.citation4
dc.identifier.issn08857490
dc.identifier.other10.1023/A:1011167023695
dc.identifier.urihttp://hdl.handle.net/10019.1/14197
dc.subjectcholesterol
dc.subjectferrous ion
dc.subjectfree radical
dc.subjectmelatonin
dc.subjectprasterone
dc.subjectprasterone sulfate
dc.subjectscavenger
dc.subjectsitogluside
dc.subjectsitosterol
dc.subjectthiobarbituric acid reactive substance
dc.subjectaging
dc.subjectAlzheimer disease
dc.subjectcell death
dc.subjectcell membrane
dc.subjectcomparative study
dc.subjectconference paper
dc.subjectcontrolled study
dc.subjectdrug structure
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectlipid peroxidation
dc.subjectthrombocyte membrane
dc.subjectviscosity
dc.subjectAging
dc.subjectAndrosterone
dc.subjectAntilipemic Agents
dc.subjectAntioxidants
dc.subjectBlood Platelets
dc.subjectCholesterol
dc.subjectFree Radical Scavengers
dc.subjectHumans
dc.subjectLipid Peroxidation
dc.subjectMelatonin
dc.subjectSitosterols
dc.titleA comparative study of the effects of cholesterol, beta-sitosterol, beta-sitosterol glucoside, dehydroepiandrosterone sulphate and melatonin on in vitro lipid peroxidation
dc.typeConference Paper
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