Molecular epidemiology of mother-to-child transmission of HIV-1 in children at Tygerberg Hospital

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dc.contributor.advisorVan Zyl, G. U.
dc.contributor.advisorEngelbrecht, S.
dc.contributor.authorKorsman, Stephen Nicolaas Jacquesen_ZA
dc.contributor.otherUniversity of Stellenbosch. Faculty of Health Sciences. Dept. of Pathology. Medical Microbiology.
dc.date.accessioned2008-07-16T09:08:48Zen_ZA
dc.date.accessioned2010-07-09T11:08:01Z
dc.date.available2008-07-16T09:08:48Zen_ZA
dc.date.available2010-07-09T11:08:01Z
dc.date.issued2006-12en_ZA
dc.descriptionThesis (MMed (Medical Microbiology))--University of Stellenbosch, 2006.
dc.description.abstractOne of the major routes of transmission of human immunodeficiency virus (HIV) in the developing world is vertical transmission from mother to infant – pre-, intra-, or post-partum. In the Western Cape, HIV-1 subtype C is the predominant subtype in the heterosexual population, and this trend was expected to be seen amongst cases of mother-to-child transmission of HIV. The aim of this study was to perform genetic characterisation and phylogenetic analysis of the HIV-1 genome in positive serum/plasma samples obtained from children (age 0 to 18 months) from 2000-2002, and temporally related specimens from their mothers. We obtained 27 suitable pairs of samples taken within 6 months of delivery. From this pool, we obtained 21 infant DNA sequences and 17 maternal sequences, resulting in 16 mother-infant pairs. All patient sequences were identified as HIV-1 subtype C, and, as expected, mother and infant viral sequences clustered together. In some cases where a mother was suspected to have two dominant quasispecies based on the electropherogram, only one sequence was detectable in the infant. Single or multiple amino acid deletions were consistent between mothers and infants, and some pairs showed the same amino acid deletions seen in other pairs.en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/3335
dc.language.isoenen_ZA
dc.publisherStellenbosch : University of Stellenbosch
dc.rights.holderUniversity of Stellenbosch
dc.subjectVerticalen_ZA
dc.subjectHIV infections -- Transmission -- South Africaen_ZA
dc.subjectHIV-positive women -- South Africaen
dc.subjectAIDS (Disease) -- Transmission -- South Africaen
dc.subjectAIDS (Disease) in infants -- South Africaen
dc.subjectAIDS (Disease) in pregnancy -- South Africaen
dc.subjectDissertations -- Medical microbiologyen
dc.subjectTheses -- Medical microbiologyen
dc.subjectAssignments -- Medical microbiologyen
dc.titleMolecular epidemiology of mother-to-child transmission of HIV-1 in children at Tygerberg Hospitalen_ZA
dc.typeThesisen_ZA
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