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- ItemAn Evaluation of High‑Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort(Springer Nature, 2024-05-06) Harbor, Sharon N.; Schneider, Johann W.; Solomons, Nadine; Sanderson, Micheline; Afrogheh, Amir H.Background To determine the prevalence of HR-HPV in a series of lip SCC from South African patients, using currently accepted HPV-testing methodologies and to define the clinical and histomorphologic features of HPV-associated lip SCC. Methods Fifty SCC of lip and 50 control cases were tested for HR-HPV using p16 and HR-HPV DNA PCR. p16-equivocal/positive and HPV DNA PCR-positive SCC were further evaluated for the expression of HPV-16 and HPV-18 mRNA transcripts using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to confirm transcriptionally active HPV. Results p16 was positive in 22% (n = 11) and equivocal in 4% (n = 2) of the SCC. One p16-positive case showed positivity for both HPV-16 DNA and HPV-16 E6/E7 mRNA transcripts (HPV prevalence rate of 2%). The HPV-positive case was non-keratinizing and occurred in an 80-year-old female. The two p16-equivocal cases were HR-HPV DNA positive and mRNA PCR negative. p16 was found to have a positive predictive value of 9%. Conclusion Findings from our cohort of lip SCC suggest that HR-HPV may have an insignificant role in the pathogenesis of SCC at this site. Due to its low ppv, p16 is insufficient to establish HR-HPV infection in SCC of the lip. The combination of p16 and DNA PCR appears to correlate with the presence of transcriptionally active virus. HPV E6/E7 mRNA detection is the gold standard for identifying HR-HPV. mRNA testing is not widely available in sub-Saharan Africa due to technical and financial constraints; however, the test appears to be of great value in p16-equivocal lip SCC.
- ItemThe impact of weevil predation on seed viability and agronomic performance of cowpeas, Vigna unguiculata L. (Walp)(2023-03) Dlamini, Welile; Wilson, Ida; Pieterse, Petrus; Swanepoel, Pieter
- ItemAn Exploration of Lower Limb Prosthetics Service Delivery in Namibia in Comparison to Global Standards(2023-03) Likando, Christopher; Mji, Gubela; Visagie, Surona
- ItemAlternative pest control methods for false codling moth, Thaumatotibia leucotreta (Lepidoptera: Tortricidae): Exploring juvenile hormone analogs and entomopathogenic nematodes for integrated pest management(2023-03) Viljoen, Nadia; Karsten, Minette; Terblanche, John; Stokwe, Nomakholwa
- ItemMetabolic control analysis using glycolytic inhibitor titrations in the MCF-7 breast cancer cell lineHollocks, Stephanie Anne; Snoep, Jacob Leendert; Van Niekerk, David Douglas