Comparative characterization and mutational analysis of type III pantothenate kinases

Date
2006-03
Authors
Brand, Leisl Anne
Journal Title
Journal ISSN
Volume Title
Publisher
Stellenbosch : University of Stellenbosch
Abstract
This thesis reports the cloning, overexpression and characterization of the coaX gene product from Bacillus subtilis and its homologue from Helicobacter pylori. It demonstrates that these proteins have pantothenate kinase activity. Compared to the two pantothenate kinase analogues classified to date, these two enzymes exhibit distinctly different characteristics, suggesting that they are the first characterized examples of a third pantothenate kinase analogue. In addition, mutational studies are presented that probe the importance of conserved aspartate residues within the active sites of these newly characterized analogues. The results show that these residues are important for the activity of the protein.
Description
Thesis (MSc (Biochemistry))--University of Stellenbosch, 2006.
Keywords
Pantothenate kinases, Dissertations -- Biochemistry, Theses -- Biochemistry, Protein kinases, Enzymes, Proteins -- Synthesis, Proteins -- Analysis
Citation