Genotypic and phenotypic analysis of the Env gene from South African HIV-1 subtype B and C isolates
Date
2002
Authors
Treurnicht F.K.
Smith T.-L.
Engelbrecht S.
Claassen M.
Robson B.A.
Zeier M.
Van Rensburg E.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultures. The amino acid charge of the V3 loop correlated with the NSI phenotype of the strains. The V3 competitive peptide enzyme immunoassay and DNA sequencing of the partial gp120 region gave concordant results on the 15 subtype C strains, whereas the three B genotypes gave a positive to B, a nonreactive to B, and a dual reaction to the B-D peptides, respectively. Sixteen of the isolates used only CCR5 as coreceptor whereas two isolates made use of additional coreceptors including CXCR4. In summary, all our subtype C isolates are NSI phenotypically and almost all of them use CCR5 exclusively as their coreceptor. © 2002 Wiley-Liss, Inc.
Description
Keywords
chemokine receptor CCR5, chemokine receptor CXCR4, DNA, glycoprotein gp 120, Human immunodeficiency virus vaccine, amino acid analysis, animal cell, article, clinical article, controlled study, correlation analysis, drug design, envelope gene, enzyme immunoassay, gene isolation, genotype, human, human cell, Human immunodeficiency virus 1, nonhuman, nucleotide sequence, patient selection, phenotype, South Africa, strain identification, vaccine production, virus identification, virus typing, Amino Acid Sequence, Cell Line, Cytopathogenic Effect, Viral, Genes, env, Genotype, Giant Cells, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Molecular Sequence Data, Peptide Fragments, Phenotype, Phylogeny, Receptors, CCR5, Receptors, CXCR4, Sequence Homology, Amino Acid, South Africa, Human immunodeficiency virus, Human immunodeficiency virus 1
Citation
Journal of Medical Virology
68
2
68
2