Evidence for the binding of the carcinogen 3 methylcholanthrene to both the purine and the pyrimidine bases of hamster fibroblast deoxyribonucleic acid
Date
1973
Authors
Jones P.A.
Gevers W.
Hawtrey A.O.
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Abstract
The binding of [3H]3 methylcholanthrene to the DNA of hamster fibroblasts was studied by using chemical methods for DNA degradation. DNA depurinated by mild acid hydrolysis released approximately 1/2 of the radioactivity at the same rate as the purine bases, but the resulting apurinic acid still contained radioactive carcinogen.
Description
Keywords
3 methylcholanthrene, dna, purine, pyrimidine, column chromatography, drug analysis, drug binding, fibroblast, in vitro study, methylcholanthrene h 3, pharmacology, theoretical study, Animal, Binding Sites, Chromatography, DNA, Fibroblasts, Hamsters, Hydrolysis, Methylcholanthrene, Purine Nucleotides, Pyrimidine Nucleotides, Tissue Culture, Tritium
Citation
Biochemical Journal
135
2
135
2