Xylose utilisation by recombinant strains of Saccharomyces cerevisiae on different carbon sources
Date
1999
Authors
Van Zyl W.H.
Eliasson A.
Hobley T.
Hahn-Hagerdal B.
Journal Title
Journal ISSN
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Abstract
Autoselective xylose-utilising strains of Saccharomyces cerevisiae expressing the xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes of Pichia stipitis were constructed by replacing the chromosomal FUR1 gene with a disrupted fur1::LEU2 allele. Anaerobic fermentations with 80 g l-1 D-xylose as substrate showed a twofold higher consumption of xylose in complex medium compared to defined medium. The xylose consumption rate increased a further threefold when 20 g l-1 D-glucose or raffinose was used as co-substrate together with 50 g l-1 D-xylose. Xylose consumption was higher with raffinose as co-substrate than with glucose (85% versus 71%, respectively) after 82 h fermentations. A high initial ethanol concentration and moderate levels of glycerol and acetic acid accompanied glucose as co- substrate, whereas the ethanol concentration gradually increased with raffinose as co-substrate with no glycerol and much less acetic acid formation.
Description
Keywords
acetic acid, alcohol, carbon, glucose, glycerol, oxidoreductase, raffinose, xylose, anaerobic metabolism, article, gene disruption, gene expression, nonhuman, pichia stipitis, saccharomyces cerevisiae, Acetic acid, Anaerobiosis, Chromatography, High Pressure Liquid, D-Xylulose Reductase, Endo-1,4-beta Xylanases, Ethanol, Fungal proteins, Glucose, Glycerol, Plasmids, Raffinose, Recombination, Genetic, Saccharomyces cerevisiae, Sugar alcohol dehydrogenases, Time factors, Xylose, Xylosidases, Pichia stipitis, Saccharomyces cerevisiae, Trixis
Citation
Applied Microbiology and Biotechnology
52
6
52
6