Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

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dc.contributor.author La Grange D.C.
dc.contributor.author Claeyssens M.
dc.contributor.author Pretorius I.S.
dc.contributor.author Van Zyl W.H.
dc.date.accessioned 2011-05-15T15:56:37Z
dc.date.available 2011-05-15T15:56:37Z
dc.date.issued 2000
dc.identifier.citation Applied Microbiology and Biotechnology
dc.identifier.citation 54
dc.identifier.citation 2
dc.identifier.issn 1757598
dc.identifier.other 10.1007/s002530000372
dc.identifier.uri http://hdl.handle.net/10019.1/9951
dc.description.abstract The xynB gene encoding the Bacillus pumilus β-xylosidase was expressed separately and jointly with the Trichoderma reesei β-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (ADH2(P)) and terminator (ADH2(T)) sequences. The xynB gene was fused in frame to the yeast mating factor α1 secretion sequence (MFα1(S)) to effect secretion in S. cerevisiae. The fusion protein was designated Xlo1. Xlo1 produced in S. cerevisiae exhibited low affinity for xylobiose, but eventually hydrolyzed xylobiose and xylotriose to the monomeric constituent, D-xylose. Coproduction of Xyn2 and Xlo1 by S. cerevisiae led to a 25% increase in the amount of reducing sugars released from birchwood xylan compared to S. cerevisiae producing only the Xyn2 β-xylanase. However, no D-xylose was produced from birchwood xylan, presumably due to very low Xlo1 β-xylosidase activity and its low affinity for xylobiose.
dc.subject alcohol dehydrogenase
dc.subject bacterial enzyme
dc.subject beta xylanase 2
dc.subject carbohydrate derivative
dc.subject glucose
dc.subject unclassified drug
dc.subject xylan
dc.subject xylan 1,4 beta xylosidase
dc.subject xylobiose
dc.subject xylose
dc.subject xylotriose
dc.subject article
dc.subject Bacillus pumilus
dc.subject enzyme activity
dc.subject enzyme analysis
dc.subject enzyme kinetics
dc.subject enzyme specificity
dc.subject gene control
dc.subject gene fusion
dc.subject genetic manipulation
dc.subject nonhuman
dc.subject nucleotide sequence
dc.subject protein expression
dc.subject Saccharomyces cerevisiae
dc.subject Trichoderma reesei
dc.subject Alcohol Dehydrogenase
dc.subject Bacillus
dc.subject Blotting, Northern
dc.subject Cloning, Molecular
dc.subject Electrophoresis, Polyacrylamide Gel
dc.subject Endo-1,4-beta Xylanases
dc.subject Gene Expression
dc.subject Promoter Regions (Genetics)
dc.subject Recombinant Proteins
dc.subject RNA, Messenger
dc.subject Saccharomyces cerevisiae
dc.subject Sequence Analysis, DNA
dc.subject Terminator Regions (Genetics)
dc.subject Trichoderma
dc.subject Xylans
dc.subject Xylose
dc.subject Xylosidases
dc.subject Bacillus pumilus
dc.subject Bacteria (microorganisms)
dc.subject Hypocrea jecorina
dc.subject Pumilus
dc.subject Saccharomyces cerevisiae
dc.subject Trichoderma
dc.subject Yamagishiella
dc.title Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
dc.type Article
dc.description.version Article


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