ITEM VIEW

Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

dc.contributor.authorLa Grange D.C.
dc.contributor.authorClaeyssens M.
dc.contributor.authorPretorius I.S.
dc.contributor.authorVan Zyl W.H.
dc.date.accessioned2011-05-15T15:56:37Z
dc.date.available2011-05-15T15:56:37Z
dc.date.issued2000
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation54
dc.identifier.citation2
dc.identifier.issn1757598
dc.identifier.other10.1007/s002530000372
dc.identifier.urihttp://hdl.handle.net/10019.1/9951
dc.description.abstractThe xynB gene encoding the Bacillus pumilus β-xylosidase was expressed separately and jointly with the Trichoderma reesei β-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (ADH2(P)) and terminator (ADH2(T)) sequences. The xynB gene was fused in frame to the yeast mating factor α1 secretion sequence (MFα1(S)) to effect secretion in S. cerevisiae. The fusion protein was designated Xlo1. Xlo1 produced in S. cerevisiae exhibited low affinity for xylobiose, but eventually hydrolyzed xylobiose and xylotriose to the monomeric constituent, D-xylose. Coproduction of Xyn2 and Xlo1 by S. cerevisiae led to a 25% increase in the amount of reducing sugars released from birchwood xylan compared to S. cerevisiae producing only the Xyn2 β-xylanase. However, no D-xylose was produced from birchwood xylan, presumably due to very low Xlo1 β-xylosidase activity and its low affinity for xylobiose.
dc.subjectalcohol dehydrogenase
dc.subjectbacterial enzyme
dc.subjectbeta xylanase 2
dc.subjectcarbohydrate derivative
dc.subjectglucose
dc.subjectunclassified drug
dc.subjectxylan
dc.subjectxylan 1,4 beta xylosidase
dc.subjectxylobiose
dc.subjectxylose
dc.subjectxylotriose
dc.subjectarticle
dc.subjectBacillus pumilus
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme kinetics
dc.subjectenzyme specificity
dc.subjectgene control
dc.subjectgene fusion
dc.subjectgenetic manipulation
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectprotein expression
dc.subjectSaccharomyces cerevisiae
dc.subjectTrichoderma reesei
dc.subjectAlcohol Dehydrogenase
dc.subjectBacillus
dc.subjectBlotting, Northern
dc.subjectCloning, Molecular
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectEndo-1,4-beta Xylanases
dc.subjectGene Expression
dc.subjectPromoter Regions (Genetics)
dc.subjectRecombinant Proteins
dc.subjectRNA, Messenger
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Analysis, DNA
dc.subjectTerminator Regions (Genetics)
dc.subjectTrichoderma
dc.subjectXylans
dc.subjectXylose
dc.subjectXylosidases
dc.subjectBacillus pumilus
dc.subjectBacteria (microorganisms)
dc.subjectHypocrea jecorina
dc.subjectPumilus
dc.subjectSaccharomyces cerevisiae
dc.subjectTrichoderma
dc.subjectYamagishiella
dc.titleCoexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
dc.typeArticle
dc.description.versionArticle


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

ITEM VIEW