Differential uptake of fumarate by Candida utilis and Schizosaccharomyces pombe

Date
2000
Authors
Saayman M.
Van Vuuren H.J.J.
Van Zyl W.H.
Viljoen-Bloom M.
Journal Title
Journal ISSN
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Abstract
The dicarboxylic acid fumarate is an important intermediate in cellular processes and also serves as a precursor for the commercial production of fine chemicals such as L-malate. Yeast species differ remarkably in their ability to degrade extracellular dicarboxylic acids and to utilise them as their only source of cartoon. In this study we have shown that the yeast Candida utilis effectively degraded extracellular fumarate and L-malate, but glucose or other assimilable carbon sources repressed the transport and degradation of these dicarboxylic acids. The transport of both dicarboxylic acids was shown to be strongly inducible by either fumarate or L-malate while kinetic studies suggest that the two dicarboxylic acids are transported by the same transporter protein. In contrast, Schizosaccharomyces pombe effectively degraded extracellular L-malate, but not fumarate, in the presence of glucose or other assimilable carbon sources. The Sch. pombe malate transporter was unable to transport fumarate, although fumarate inhibited the uptake of L-malate.
Description
Keywords
carbon, dicarboxylic acid, fumaric acid, glucose, malic acid, article, biodegradation, Candida utilis, ion transport, kinetics, nonhuman, Schizosaccharomyces pombe, species difference, Biological Transport, Active, Candida, Culture Media, Fumarates, Gene Expression Regulation, Fungal, Malates, Recombination, Genetic, Saccharomyces cerevisiae, Schizosaccharomyces
Citation
Applied Microbiology and Biotechnology
54
6