Procedures involving liquid media for detection of bacterial contamination in breweries
Date
1977
Authors
Van Vuuren H.J.J.
Louw H.A.
Loos M.A.
Meisel R.
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Journal ISSN
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Abstract
The liquid equivalent of universal beer agar, designated universal beer liquid medium, and its beer-free equivalent, universal liquid medium (UL), were equally effective in demonstrating bacterial contamination in 120 of 200 samples from different stages of a commercial brewing process. Growth of the contaminants after 3 days was consistently more luxuriant in the UL medium. A yeast-water substrate medium failed to reveal many contaminants detected with UL in 392 samples from three breweries and revealed only a few not detected with UL. The use of UL and a lactose-peptone medium, with microscope examination of the media for bacterial growth, permitted detection of 93% of the known contaminants compared to 87% detected with UL alone; this combination or universal beer liquid medium plus lactose-peptone medium can therefore be recommended for the detection of bacterial contaminants in brewery samples. Bacterial contamination of pitching yeasts appeared to be a particular problem in the breweries investigated.
Description
Keywords
bacterium contamination, beer, in vitro study, methodology, microorganism, article, bacterium, comparative study, culture medium, isolation and purification, lactose, peptone, Bacteria, Beer, Comparative Study, Culture Media, Evaluation Studies, Lactose, Peptones
Citation
Applied and Environmental Microbiology
33
2
33
2