Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

Southgate V.J. ; Steyn A.J.C. ; Pretorius I.S. ; Van Vuuren H.J.J. (1993)


Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.

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