Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood
Date
1998
Authors
Janse B.J.H.
Gaskell J.
Akhtar M.
Cullen D.
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Abstract
Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.The precise roles and interactions of these genes in lignin degradation and in commercial processes such as biomechanical pulping are poorly understood. Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.
Description
Keywords
Enzymes, Lignin, Manganese compounds, Regression analysis, RNA, Wood, Biomechanical pulping, Glyoxal oxidase, Lignin peroxidases, Magnetic capture, Manganese peroxidases, Phanerochaete chrysosporium, Transcript patterns, Genes, glyoxal oxidase, lignin peroxidase, manganese peroxidase, messenger RNA, polyadenylated RNA, unclassified drug, article, gene expression, magnetic field, nonhuman, Phanerochaete, polymerase chain reaction, quantitative diagnosis, soil pollution, wood, Actinobacteria (class), Chrysosporium, Phanerochaete, Phanerochaete chrysosporium, uncultured actinomycete
Citation
Applied and Environmental Microbiology
64
9
64
9