Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
Date
2001
Authors
Traff K.L.
Cordero R.R.O.
Van Zyl W.H.
Hahn-Hagerdal B.
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Abstract
Saccharomyces cerevisiae ferments hexoses efficiently but is unable to ferment xylose. When the bacterial enzyme xylose isomerase (XI) from Thermus thermophilus was produced in S. cerevisiae, xylose utilization and ethanol formation were demonstrated. In addition, xylitol and acetate were formed. An unspecific aldose reductase (AR) capable of reducing xylose to xylitol has been identified in S. cerevisiae. The GRE3 gene, encoding the AR enzyme, was deleted in S. cerevisiae CEN.PK2-1C, yielding YUSM1009a. XI from T. thermophilus was produced, and endogenous xylulokinase from S. cerevisiae was overproduced in S. cerevisiae CEN.PK2-1C and YUSM1009a. In recombinant strains from which the GRE3 gene was deleted, xylitol formation decreased twofold. Deletion of the GRE3 gene combined with expression of the xylA gene from T. thermophilus on a replicative plasmid generated recombinant xylose utilizing S. cerevisiae strain TMB3102, which produced ethanol from xylose with a yield of 0.28 mmol of C from ethanol/mmol of C from xylose. None of the recombinant strains grew on xylose.
Description
Keywords
aldehyde reductase, Aldose Ketose Isomerases, glucose, isomerase, oxygen, phosphotransferase, xylose, xylose isomerase, xylulokinase, anaerobic growth, article, enzymology, gene deletion, genetic recombination, genetics, growth, development and aging, metabolism, Saccharomyces cerevisiae, Aldehyde Reductase, Aldose-Ketose Isomerases, Anaerobiosis, Gene Deletion, Glucose, Oxygen, Phosphotransferases (Alcohol Group Acceptor), Recombination, Genetic, Saccharomyces cerevisiae, Support, Non-U.S. Gov't, Xylose
Citation
Applied and Environmental Microbiology
67
12
67
12