Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility
CITATION: Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.
The original publication is available at http://jcm.asm.org
We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.