Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD

van Schalkwyk, Frederick Josephus (Erick) (2016-03)

Thesis

ENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and progesterone as substrates ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine CYP17 ; how the time-dependent metabolism of pregnenolone, 17α-hydroxypregnenolone and dehydroepiandrosterone by ovine 3β-hydroxysteroid dehydrogenase (3β-HSD), was obtained ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine 3β-HSD

AFRIKAANSE OPSOMMING : Hierdie studie beskryf: hoe tyd-afhanklike eksperimentele data van die reaksies wat gekataliseer word deur skaap sitochroom P450 17α-hidroksielase/17,20-liase (CYP17) met pregnenoloon en progesteroon as substraat, verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap CYP17 gekataliseer word ; hoe die tyd-afhanklike metabolisme van pregnenoloon, progesteroon en dehidroepiandrosteroon wat gekataliseer word deur skaap 3β-hidroksiesteroïd dehidroginase (3β-HSD), verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap 3β-HSD gekataliseer word

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/98727
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