Development of a doubled haploid mapping population and linkage map for the bread wheat cross Kariega x Avocet S

Prins R. ; Ramburan V.P. ; Pretorius Z.A. ; Boyd L.A. ; Boshoff W.H.P. ; Smith P.H. ; Louw J.H. (2005)


A doubled haploid wheat population of 150 lines was constructed from the F1 of a cross between cultivars Kariega and Avocet S using the wheat-maize technique. Segregation and linkage analysis of 203 DNA markers, two storage protein markers and the stem rust resistance gene Sr26 yielded 31 linkage groups of which 28 were chromosome-anchored. These linkage groups covered the entire genome of 21 chromosomes. SSR markers were advantageous for chromosome anchoring and AFLP markers for consolidation of the various linkage groups. A significant feature of the linkage map is a relatively low level of polymorphism for markers on the D genome, viz, 18.5% of all markers mapped. The Kariega X Avocet S doubled haploid population and linkage maps have emerged as valuable resources for further genetic studies of qualitative and quantitative traits of economic importance in bread wheat.

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