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Simple silicone chamber system for in vitro three-dimensonal skeletal muscle tissue formation

dc.contributor.authorSnyman, Celiaen_ZA
dc.contributor.authorGoetsch, Kyle P.en_ZA
dc.contributor.authorMyburgh, Kathryn H.en_ZA
dc.contributor.authorNiesler, Carola U.en_ZA
dc.date.accessioned2014-07-06T17:36:15Z
dc.date.available2014-07-06T17:36:15Z
dc.date.issued2013-11-28
dc.identifier.citationSnyman, C., Goetsch, K. P., Myburgh, K. H. & Niesler, C. U. 2013. Simple silicone chamber system for in vitro three-dimensonal skeletal muscle tissue formation. Frontiers in Physiology, 4:1-6, doi:10.3389/fphys.2013.00349.
dc.identifier.issn1664-042X (online)
dc.identifier.otherdoi:10.3389/fphys.2013.00349
dc.identifier.urihttp://hdl.handle.net/10019.1/90779
dc.descriptionCITATION: Snyman, C., Goetsch, K. P., Myburgh, K. H. & Niesler, C. U. 2013. Simple silicone chamber system for in vitro three-dimensonal skeletal muscle tissue formation. Frontiers in Physiology, 4:1-6, doi:10.3389/fphys.2013.00349.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.description.abstractBioengineering skeletal muscle often requires customized equipment and intricate casting techniques. One of the major hurdles when initially trying to establish in vitro tissue engineered muscle constructs is the lack of consistency across published methodology. Although this diversity allows for specialization according to specific research goals, lack of standardization hampers comparative efforts. Differences in cell type, number and density, variability in matrix and scaffold usage as well as inconsistency in the distance between and type of adhesion posts complicates initial establishment of the technique with confidence. We describe an inexpensive, but readily adaptable silicone chamber system for the generation of skeletal muscle constructs that can readily be standardized and used to elucidate myoblast behavior in a three-dimensional space. Muscle generation, regeneration and adaptation can also be investigated in this model, which is more advanced than differentiated myotubes.
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fphys.2013.00349/full
dc.format.extent6 pages
dc.language.isoen
dc.publisherFrontiers
dc.subjectTissue engineeringen_ZA
dc.subjectMusculoskeletal systemen_ZA
dc.titleSimple silicone chamber system for in vitro three-dimensonal skeletal muscle tissue formationen_ZA
dc.typeArticle
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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