Now showing items 1-5 of 5
Expression behaviour of primary carbon metabolism genes during sugarcane culm development
(Stellenbosch : University of Stellenbosch, 2004-04)
ENGLISH ABSTRACT: Despite numerous attempts involving a variety of target genes, the successful transgenic manipulation of sucrose accumulation in sugarcane remains elusive. It is becoming increasingly apparent that ...
Characterization of transgenic sugarcane lines with perturbed pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity
(Stellenbosch : University of Stellenbosch, 2009-03)
Pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) is an important glycolytic enzyme and catalyses the reversible conversion of fructose-6-phosphate (Fr-6-P) and pyrophosphate (PPi) to fructose 1,6-bisphosphate ...
The manipulation of fructose 2,6-bisphosphate levels in sugarcane
(Stellenbosch : University of Stellenbosch, 2006-03)
Fructose 2,6-bisphosphate (Fru 2,6-P2) is an important regulatory molecule in plant carbohydrate metabolism. There were three main objectives in this study. Firstly, to determine whether the recombinant rat 6-phosphofructo ...
Carbon turnover and sucrose metabolism in the culm of transgenic sugarcane producing 1-Kestose
(Stellenbosch : University of Stellenbosch, 2007-12)
Carbon partitioning was investigated in sugarcane (Saccharum spp. hybrids) that was genetically modified with sucrose: sucrose 1-fructosyltransferase (1-SST; EC 184.108.40.206) from Cynara scolymus. This enzyme catalyses the ...
Genetic manipulation of sucrose-storing tissue to produce alternative products
(Stellenbosch : University of Stellenbosch, 2007-03)
The main aim of the work presented in this dissertation was to explore the possibility to genetically manipulate the sucrose storing crops, sugarcane and sweet sorghum, to convert their sucrose reserves into higher-value ...