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Characterization of a novel α-amylase from Lipomyces kononenkoae and expression of its gene (LKA1) in Saccharomyces cerevisiae

Steyn A.J.C. Pretorius I.S. (1995)

A highly active α-amylase (76 250 Da) secreted by the raw starch-degrading yeast Lipomyces kononenkoae strain IGC4052B was purified and characterized. Using high performance liquid chromatography (HPLC), end-product analysis ...
Cloning and characterization of a second α-amylase gene (LKA2) from Lipomyces kononenkoae IGC4052B and its expression in Saccharomyces cerevisiae

Eksteen J.M. Steyn A.J.C. van Rensburg P. Cordero Otero R.R. Pretorius I.S. (2003)

Lipomyces kononenkoae secretes a battery of highly effective amylases (i.e. α-amylase, glucoamylase, isoamylase and cyclomaltodextrin glucanotransferase activities) and is therefore considered as one of the most efficient ...
Development and assessment of a recombinant Saccharomyces cerevisiae wine yeast producing two aroma-enhancing β-glucosidases encoded by the Saccharomycopsis fibuligera BGL1 and BGL2 genes

Van Rensburg P. Stidwell T. Lambrechts M.G. Cordero Otero R. Pretorius I.S. (2005)

The distinctive varietal flavour of grapes and wine is affected by the absolute and relative concentrations of many compounds, including monoterpene alcohols such as citronellol, geraniol, hotrienol, linalool, nerol and ...
Development of a screening method for the identification of a novel Saccharomyces cerevisiae mutant over-expressing Trichoderma reesei cellobiohydrolase II

Gundllapalli S.B. Otero R.R.C. Pretorius I.S. (2006)

In a previous study we showed that the fusion of the cellulose-binding domain (CBD2) from Trichoderma reesei cellobiohydrolase II to a β-glucosidase (BGL1) enzyme from Saccharomycopsis fibuligera significantly hindered its ...
Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains

Du Plessis L. Rose S.H. Van Zyl W.H. (2010)

The endoglucanase I and II genes (egI or Cel7B and egII or Cel5A) of Trichoderma reesei QM6a were successfully cloned and expressed in Saccharomyces cerevisiae under the transcriptional control of the yeast ENO1 promoter ...