Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae

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Show simple item record La Grange, Daniel C. Pretorius, Isak S. Van Zyl, Willem H. 2011-04-07T08:27:35Z 2011-04-07T08:27:35Z 1995-04
dc.identifier.citation La Grange, Daniel C., Pretorius, Isak S., Van Zyl, Willem H. 1996. Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 62(3):1036-1044, en_ZA
dc.identifier.issn 1098-5336 (Online Version)
dc.identifier.issn 0099-2240 (Print Version)
dc.description Includes bibliography
dc.description.abstract The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 699-bp open reading frame that encodes a 223-amino-acid propeptide. The XYN2 gene, located on URA3-based multicopy shuttle vectors, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the alcohol dehydrogenase II (ADH2) and phosphoglycerate kinase (PGK1) gene promoters and terminators, respectively. The 33-amino-acid leader peptide of the Xyn2 beta-xylanase was recognized and cleaved at the Kex2-like Lys-Arg residues, enabling the efficient secretion and glycosylation of the heterologous beta-xylanase. The molecular mass of the recombinant beta-xylanase was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 27 kDa. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of the URA3-based XYN2 shuttle vectors in nonselective complex medium. These autoselective S. cerevisiae strains produced 1,200 and 160 nkat of beta-xylanase activity per ml under the control of the ADH2 and PGK1 promoters in rich medium, respectively. The recombinant enzyme showed highest activity at pH 6 and 60 degrees C and retained more than 90% of its activity after 60 min at 50 degrees C. en_ZA
dc.format.extent p. 1036–1044 : ill.
dc.language.iso en_US en_ZA
dc.publisher American Society for Microbiology en_ZA
dc.subject Gene expression en_ZA
dc.subject Saccharomyces cerevisiae -- Genetics en_ZA
dc.subject Xylanases en_ZA
dc.subject Recombinant en_ZA
dc.subject Trichoderma reesei en_ZA
dc.subject Xylan en_ZA
dc.subject Breakdown en_ZA
dc.title Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae en_ZA
dc.type Article en_ZA
dc.rights.holder American Society for Microbiology en_ZA
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