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Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

dc.contributor.authorLa Grange, Daniel C.
dc.contributor.authorClaeyssens, Mark
dc.contributor.authorPretorius, Isak S.
dc.contributor.authorVan Zyl, Willem H.
dc.date.accessioned2011-04-07T08:27:03Z
dc.date.available2011-04-07T08:27:03Z
dc.date.issued1999-12
dc.identifier.citationLa Grange, D.C., Claeyssens, M., Pretorius, I.S., Van Zyl, W.H. 2000. Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 54:195-200, http://www.springerlink.com.ez.sun.ac.za/content/wq93njaeewb98rdm/fulltext.pdf, DOI: 10.1007/s002530000372.en_ZA
dc.identifier.issn0175-7598 (Print Version)
dc.identifier.otherDOI: 10.1007/s002530000372
dc.identifier.urihttp://hdl.handle.net/10019.1/8488
dc.descriptionThe original publication is available at www.springerlink.com.
dc.descriptionIncludes bibliography.
dc.description.abstractThe xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (ADH2p) and terminator (ADH2T) sequences. The xynB gene was fused in frame to the yeast mating factor alpha1 secretion sequence (MFalpha1s) to effect secretion in S. cerevisiae. The fusion protein was designated Xlo1. Xlo1 produced in S. cerevisiae exhibited low affinity for xylobiose, but eventually hydrolyzed xylobiose and xylotriose to the monomeric constituent, D-xylose. Coproduction of Xyn2 and Xlo1 by S. cerevisiae led to a 25% increase in the amount of reducing sugars released from birchwood xylan compared to S. cerevisiae producing only the Xyn2 beta-xylanase. However, no D-xylose was produced from birchwood xylan, presumably due to very low Xlo1 beta-xylosidase activity and its low affinity for xylobiose.en_ZA
dc.format.extentp. 195-200 : ill.
dc.language.isoen_ZAen_ZA
dc.publisherSpringer-Verlagen_ZA
dc.subjectBacillusen_ZA
dc.subjectPumilusen_ZA
dc.subjectTrichoderma reeseien_ZA
dc.subjectXylosidaseen_ZA
dc.subjectXylanaseen_ZA
dc.subjectAssaysen_ZA
dc.subjectXyloseen_ZA
dc.subjectNitrophenylen_ZA
dc.subjectDNSen_ZA
dc.subjectXylanen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.titleCoexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiaeen_ZA
dc.typeArticleen_ZA
dc.rights.holderSpringer-Verlagen_ZA


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