Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

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dc.contributor.author La Grange, Daniel C.
dc.contributor.author Claeyssens, Mark
dc.contributor.author Pretorius, Isak S.
dc.contributor.author Van Zyl, Willem H.
dc.date.accessioned 2011-04-07T08:27:03Z
dc.date.available 2011-04-07T08:27:03Z
dc.date.issued 1999-12
dc.identifier.citation La Grange, D.C., Claeyssens, M., Pretorius, I.S., Van Zyl, W.H. 2000. Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 54:195-200, http://www.springerlink.com.ez.sun.ac.za/content/wq93njaeewb98rdm/fulltext.pdf, DOI: 10.1007/s002530000372. en_ZA
dc.identifier.issn 0175-7598 (Print Version)
dc.identifier.other DOI: 10.1007/s002530000372
dc.identifier.uri http://hdl.handle.net/10019.1/8488
dc.description The original publication is available at www.springerlink.com.
dc.description Includes bibliography.
dc.description.abstract The xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (ADH2p) and terminator (ADH2T) sequences. The xynB gene was fused in frame to the yeast mating factor alpha1 secretion sequence (MFalpha1s) to effect secretion in S. cerevisiae. The fusion protein was designated Xlo1. Xlo1 produced in S. cerevisiae exhibited low affinity for xylobiose, but eventually hydrolyzed xylobiose and xylotriose to the monomeric constituent, D-xylose. Coproduction of Xyn2 and Xlo1 by S. cerevisiae led to a 25% increase in the amount of reducing sugars released from birchwood xylan compared to S. cerevisiae producing only the Xyn2 beta-xylanase. However, no D-xylose was produced from birchwood xylan, presumably due to very low Xlo1 beta-xylosidase activity and its low affinity for xylobiose. en_ZA
dc.format.extent p. 195-200 : ill.
dc.language.iso en_ZA en_ZA
dc.publisher Springer-Verlag en_ZA
dc.subject Bacillus en_ZA
dc.subject Pumilus en_ZA
dc.subject Trichoderma reesei en_ZA
dc.subject Xylosidase en_ZA
dc.subject Xylanase en_ZA
dc.subject Assays en_ZA
dc.subject Xylose en_ZA
dc.subject Nitrophenyl en_ZA
dc.subject DNS en_ZA
dc.subject Xylan en_ZA
dc.subject Saccharomyces cerevisiae en_ZA
dc.title Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae en_ZA
dc.type Article en_ZA
dc.rights.holder Springer-Verlag en_ZA


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