The use of the polymerase chain reaction test in the diagnosis of tuberculosis

Van Helden P.D. ; Du Toit R. ; Jordaan A. ; Taljaard B. ; Pitout J. ; Victor T. (1991)


The original publication is available at


Current techniques for laboratory diagnosis of tuberculosis have some serious limitations. These include the high cost and time required for the current assays. The development of a rapid, sensitive, specific and low-cost assay is therefore of considerable importance. We report here the development and laboratory testing of a polymerase chain reaction DNA-based diagnostic test for the presence of Mycobacterium tuberculosis in sputum. The assay shows a high level of sensitivity and specificity and requires considerably less capital, consumables and time inputs than existing laboratory tests. We believe this technology is ready for large-scale evaluation and use, particularly in hospital-based laboratories.

Please refer to this item in SUNScholar by using the following persistent URL:
This item appears in the following collections: