Browsing Masters Degrees (Genetics) by Title
Now showing items 46-65 of 208
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The detection of Citrus tristeza virus genetic variants using pathogen specific electronic probes
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Citrus tristeza virus (CTV), a complex pathogen of citrus spp., is endemic to South Africa and has been responsible for great losses locally and internationally. CTV causes severe stem pitting in grapefruit, ... -
The detection of mycoviral sequences in grapevine using next-generation sequencing
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Metagenomic studies that make use of next-generation sequencing (NGS) generate large amounts of sequence data, representing the genomes of multiple organisms of which no prior knowledge is necessarily ... -
Detection of sequence diversity in the CYP2C19 gene of Xhosa South African individuals : an analytical and comparative study including in silico and functional analysis of the 5’ flanking region
(Stellenbosch : University of Stellenbosch, 2010-03)ENGLISH ABSTRACT: The prevalence of adverse drug reactions (ADR) and treatment failure in South Africa requires urgent addressing and it is the aim of pharmacogenetics to aid in the alleviation of these ADRs and treatment ... -
The determination of the spatial and temporal distribution of Aster Yellows phytoplasma in grapevine
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: South Africa is ranked amongst the top ten for wine production internationally. Viticulture contributes immensely to the economy, which justifies research into the pathogens that may negatively affect ... -
Determination of the virus diversity associated with Grapevine leafroll disease
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: Vitis vinifera is the woody crop most susceptible to intracellular pathogens. Currently 70 pathogens infect grapevine, of which 63 are of viral origin. Grapevine leafroll-associated virus 3 (GLRaV-3) is ... -
The development of a diagnostic assay for nepoviruses in grapevine
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: The nepoviruses are a group of nematode-transmitted plant viruses that are distributed worldwide and infect a wide range of plant species, including grapevine. Most of the nepoviruses are foreign to ... -
Development of a hairy root bioreactor from Stevia rebaudiana to produce steviol glycosides
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH SUMMARY: Extracts from the leaves of Stevia rebaudiana, a plant native to South America, have been used as natural sweetener for centuries. With the global epidemic of obesity linked to increased prevalence of ... -
Development of AFLP markers for Haliotis midae for linkage mapping
(Stellenbosch : Stellenbosch University, 2008-12)ENGLISH ABSTRACT: Haliotis midae, is the only commercially important species of the six abalone species found in South African coastal waters and has become a lucrative commercial commodity. Wild stocks of H. midae are, ... -
Development of alternative diagnostic protocols for Candidatus Phytoplasma asteris and Coniella granati Sacc. (Syn. Pilidiella granati)
(Stellenbosch : Stellenbosch University, 2019-04)ENGLISH ABSTRACT: The Republic of South Africa (RSA) is an integral part of the global fruit exporting chain. Currently, South Africa is ranked eighth in the world in wine production, exporting 40% of all locally produced ... -
The development of an enzyme linked immunosorbent assay for the detection of the South African strain(s) of grapevine fanleaf nepovirus
(Stellenbosch : Stellenbosch University, 2008-12)South Africa is one of the top ten wine producing countries in the world. The South African wine industry contributes approximately R16.3 billion to South Africa’s annual gross domestic product with 42.8% of wine being ... -
Development of gene-linked molecular markers in South African abalone (Haliotis midae) using an in silico mining approach
(Stellenbosch : University of Stellenbosch, 2010-03)ENGLISH ABSTRACT: The South African abalone, Haliotis midae, is the only endemic species of commercial value. Aquaculture remains the only avenue for expanding the industry, since the closure of the fishery. The current ... -
Development of in situ hybridisation to examine tissue-specific expression patterns of the invertase genes in sugarcane culm
(Stellenbosch : University of Stellenbosch, 2005-04)ENGLISH ABSTRACT: The goals of this project were firstly to develop the tissue preparation and in situ hybridisation protocols for sugarcane culm tissue, and secondly to use the developed techniques to examine the ... -
The development, validation and implementation of a drought stress index for the evaluation of the drought tolerance potential of South African sugarcane
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: In the rainfed areas of the South African sugar industry the unpredictability of rainfall is of major concern for producers. Currently, research into the drought tolerance of South African sugarcane ... -
A diallel study of Secale cereale
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Rye (Secale cereale) originated and was domesticated in the fertile cresent in the Middel East. It has been part of the human staple diet for thousands of years in those areas as well as Eastern Europe. ... -
Differential gene expression during berry ripening in Vitis vinifera (cv Chardonnay) : isolation of specific sequences through subtractive cloning
(Stellenbosch : Stellenbosch University, 2002-12)ENGLISH ABSTRACT: Grapevine is worldwide an agronomically important crop. Traditionally selective breeding has been used to improve existing cultivars. In the last ten years, however, the advent of biotechnology has ... -
Effect of genetic variants in genes encoding two nuclear receptors (PXR and CAR) on efavirenz levels and treatment outcome in South African HIV-infected females
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Efavirenz is an antiretroviral drug used in the treatment of HIV-positive patients as part of first line triple-highly active antiretroviral therapy. Treatment response varies among individuals and adverse ... -
The effect of triploidy on the growth and survival of the indigenous abalone, Haliotis midae, over a 24 month period under commercial rearing conditions
(Stellenbosch : Stellenbosch University, 2008-03)ENGLISH ABSTRACT: Triploidy is the genetic state of containing three sets of chromosomes per cell in stead of two as in diploid organisms. The South African abalone (Haliotis midae) is naturally a diploid organism that ... -
The effects of the synthetic strigolactone GR24 on Arabidopsis thaliana callus culture
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Plant growth promoting substances (PGPS) are emerging as useful tools in the investigation of important plant growth traits. Two PGPS, smoke-water derived from burning plant material and a synthetic ... -
The efficacy of the antimicrobial peptides D4E1, VvAMP-1 and Snakin1 against the grapevine pathogen aster yellows phytoplasma
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Phytoplasma diseases have caused disastrous effects in vineyards around the world. Therefore, the recent discovery of phytoplasmas in South African vineyards could be highly detrimental to the local ... -
Elucidation of the biochemical mechanism of glycogen phosphorylation in Escherichia coli
(Stellenbosch : University of Stellenbosch, 2009-12)ENGLISH ABSTRACT: Glycogen was isolated from E. coli and analysed for the amount of phosphate present within it. It was confirmed that a significant proportion of the glucose residues were phosphorylated at the C6 position. ...
