Identification of lactic acid bacteria isolated from the urogenital tract of women

Silvester, Marieka Elizma (1999-12)

Thesis (M.Sc.) -- University of Stellenbosch, 1999.

Thesis

ENGLISH SUMMARY: Lactic acid bacteria are present in the oral cavity, gastrointestinal tract, vagina, plants, fermented foods, manure and sewage (Kandler & Weiss, 1986). These organisms are acidophilic and thrive at a low pH which renders the environment unsuitable for most other micro-organisms. A number of lactic acid bacteria (LAB) have probiotic properties, e.g. some strains in the gastrointestinal tract inhibit the growth of Helicobacter spp. which causes stomach ulcers (Midolo et a/., 1995). Some probiotic Lactobacillus spp. may also play a role in cholesterol reduction (Mital & Garg, 1995). A few reports have been published on the role of LAB in the prevention of preterm delivery and the protection against bacterial vaginosis (Hillier et al., 1992; Martius et al., 1988; Shalev et al., 1995). A number of species produce antimicrobial peptides that inhibit the growth of other microbes, including potential pathogens. Strains thus far isolated from the human vagina are Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus casei (Rogosa & Sharpe, 1960; Giorgi et al., 1987). In most of these studies simple biochemical and phenotypical tests were used. The purpose of this study was to isolate and identify lactic acid bacteria isolated from the posterior fornix of the vagina of non-pregnant and pregnant patients. Advanced taxonomic methods were used, e.g. sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE), random amplified polymorphic DNA (RAPD)-PCR and 16S rRNA sequencing.

AFRIKAANSE OPSOMMING: Melksuurbakteriee kom voor in die mond, spysverteringskanaal, vagina, plante, gefermenteerde voedsel en riool (Kandler & Weiss, 1986). Hierdie mikro-organismes is asidofilies en floreer by 'n lae pH wat die omgewing ongeskik maak vir meeste ander mikroorganismes. Sommige melksuurbakteriee het probiotiese eienskappe, byvoorbeeld sommige stamme in die spysverteringskanaal inhibeer die groei van Helicobacter spp. wat maagsere veroorsaak (Midolo et al., 1995). Sommige melksuurbakteriee mag dalk 'n rol speel in die verlaging van cholestrolvlakke (Mital & Garg, 1995). Die rol van melksuurbakteriee in die voorkoming van vroeggebore babas en die beskerming teen bakteriese vaginose is reeds beskryf (Hillier et al., 1992; Martius et al., 1988; Shalev et al., 1995). Sommige stamme produseer antimikrobiese peptiede wat die groei van ander mikrobe, insluitend potensiele patogene, inhibeer. Tot dusver is Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus plantarum en Lactobacillus casei uit die menslike vagina ge'isoleer (Rogosa & Sharpe, 1960; Giorgi et al., 1987). In die meeste van hierdie studies is van biochemiese- of fenotipiese metodes gebruik gemaak. Die doel van hierdie studie was om melksuurbakteriee uit die vagina van swanger en nie-swanger pasiente te isoleer en te identifiseer. Gevorderde taksonomiese metodes is gebruik, naamlik numeriese analiese van SDS-poliakrielamied jelelektroforese (SDS-PAGE), lukraak ge-amplifiseerde polimorfiese DNA (RAPD)-polimerase kettingreaksie (PKR) en 16S rRNA volgordebepaling.

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