Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair

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dc.contributor.author Karboul, Anis
dc.contributor.author Gey van Pittius, Nicolaas C.
dc.contributor.author Namouchi, Amine
dc.contributor.author Vincent, Veronique
dc.contributor.author Sola, Christophe
dc.contributor.author Rastogi, Nalin
dc.contributor.author Suffys, Philip
dc.contributor.author Fabre, Michel
dc.contributor.author Cataldi, Angel
dc.contributor.author Huard, Richard C.
dc.contributor.author Kurepina, Natalia
dc.contributor.author Kreiswirth, Barry
dc.contributor.author Ho, John L.
dc.contributor.author Gutierrez, M. Cristina
dc.contributor.author Mardassi, Helmi
dc.date.accessioned 2010-12-14T13:26:13Z
dc.date.available 2010-12-14T13:26:13Z
dc.date.issued 2006-12
dc.identifier.citation Karboul, A, Gey van Pittius, NC, Namouchi, A, Vincent, V, Sola, C, Rastogi, N, Suffys, P, Fabre, M, Cataldi, A, Huard, RC, Kurepina, N, Kreiswirth, B, Ho, JL, Gutierrez, MC & Mardassi, H 2006, 'Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair', BMC Evolutionary Biology, 6(1):107. en_ZA
dc.identifier.issn 1471-2148
dc.identifier.other http://dx.doi.org/10.1186/1471-2148-6-107
dc.identifier.uri http://hdl.handle.net/10019.1/5117
dc.description.abstract Background: The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacteria where they seem to have expanded as a result of gene duplication events. PE_PGRS genes are characterized by their high GC content and extensive repetitive sequences, making them prone to recombination events and genetic variability. Results: Comparative sequence analysis of Mycobacterium tuberculosis genes PE_PGRS17 (Rv0978c) and PE_PGRS18 (Rv0980c) revealed a striking genetic variation associated with this typical tandem duplicate. In comparison to the M. tuberculosis reference strain H37Rv, the variation (named the 12/40 polymorphism) consists of an in-frame 12-bp insertion invariably accompanied by a set of 40 single nucleotide polymorphisms (SNPs) that occurs either in PE_PGRS17 or in both genes. Sequence analysis of the paralogous genes in a representative set of worldwide distributed tubercle bacilli isolates revealed data which supported previously proposed evolutionary scenarios for the M. tuberculosis complex (MTBC) and confirmed the very ancient origin of "M. canettii" and other smooth tubercle bacilli. Strikingly, the identified polymorphism appears to be coincident with the emergence of the post-bottleneck successful clone from which the MTBC expanded. Furthermore, the findings provide direct and clear evidence for the natural occurrence of gene conversion in mycobacteria, which appears to be restricted to modern M. tuberculosis strains. Conclusion: This study provides a new perspective to explore the molecular events that accompanied the evolution, clonal expansion, and recent diversification of tubercle bacilli. en_ZA
dc.language.iso en_ZA en_ZA
dc.publisher BioMed Central en_ZA
dc.subject PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes en_ZA
dc.subject Mycobacterium tuberculosis en_ZA
dc.subject Tuberculosis -- Research en_ZA
dc.subject Pathogenic bacteria en_ZA
dc.title Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair en_ZA
dc.type Article en_ZA
dc.date.updated 2010-11-09T12:55:21Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder Karboul et al.; licensee BioMed Central Ltd. en_ZA


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