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dc.contributor.authorKarboul, Anis
dc.contributor.authorGey van Pittius, Nicolaas C.
dc.contributor.authorNamouchi, Amine
dc.contributor.authorVincent, Veronique
dc.contributor.authorSola, Christophe
dc.contributor.authorRastogi, Nalin
dc.contributor.authorSuffys, Philip
dc.contributor.authorFabre, Michel
dc.contributor.authorCataldi, Angel
dc.contributor.authorHuard, Richard C.
dc.contributor.authorKurepina, Natalia
dc.contributor.authorKreiswirth, Barry
dc.contributor.authorHo, John L.
dc.contributor.authorGutierrez, M. Cristina
dc.contributor.authorMardassi, Helmi
dc.date.accessioned2010-12-14T13:26:13Z
dc.date.available2010-12-14T13:26:13Z
dc.date.issued2006-12
dc.identifier.citationKarboul, A, Gey van Pittius, NC, Namouchi, A, Vincent, V, Sola, C, Rastogi, N, Suffys, P, Fabre, M, Cataldi, A, Huard, RC, Kurepina, N, Kreiswirth, B, Ho, JL, Gutierrez, MC & Mardassi, H 2006, 'Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair', BMC Evolutionary Biology, 6(1):107.en_ZA
dc.identifier.issn1471-2148
dc.identifier.otherhttp://dx.doi.org/10.1186/1471-2148-6-107
dc.identifier.urihttp://hdl.handle.net/10019.1/5117
dc.description.abstractBackground: The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacteria where they seem to have expanded as a result of gene duplication events. PE_PGRS genes are characterized by their high GC content and extensive repetitive sequences, making them prone to recombination events and genetic variability. Results: Comparative sequence analysis of Mycobacterium tuberculosis genes PE_PGRS17 (Rv0978c) and PE_PGRS18 (Rv0980c) revealed a striking genetic variation associated with this typical tandem duplicate. In comparison to the M. tuberculosis reference strain H37Rv, the variation (named the 12/40 polymorphism) consists of an in-frame 12-bp insertion invariably accompanied by a set of 40 single nucleotide polymorphisms (SNPs) that occurs either in PE_PGRS17 or in both genes. Sequence analysis of the paralogous genes in a representative set of worldwide distributed tubercle bacilli isolates revealed data which supported previously proposed evolutionary scenarios for the M. tuberculosis complex (MTBC) and confirmed the very ancient origin of "M. canettii" and other smooth tubercle bacilli. Strikingly, the identified polymorphism appears to be coincident with the emergence of the post-bottleneck successful clone from which the MTBC expanded. Furthermore, the findings provide direct and clear evidence for the natural occurrence of gene conversion in mycobacteria, which appears to be restricted to modern M. tuberculosis strains. Conclusion: This study provides a new perspective to explore the molecular events that accompanied the evolution, clonal expansion, and recent diversification of tubercle bacilli.en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.subjectPE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genesen_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectTuberculosis -- Researchen_ZA
dc.subjectPathogenic bacteriaen_ZA
dc.titleInsights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pairen_ZA
dc.typeArticleen_ZA
dc.date.updated2010-11-09T12:55:21Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderKarboul et al.; licensee BioMed Central Ltd.en_ZA


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