Browsing Research Articles (Microbiology) by Title
Now showing items 9-28 of 100
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Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant
(SpringerOpen, 2017)Biosurfactants are unique secondary metabolites, synthesised non-ribosomally by certain bacteria, fungi and yeast, with their most promising applications as antimicrobial agents and surfactants in the medical and food ... -
Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates
(BioMed Central, 2005-05)Microorganisms are used in large-scale heap or tank aeration processes for the commercial extraction of a variety of metals from their ores or concentrates. These include copper, cobalt, gold and, in the past, uranium. The ... -
Characterization of killer yeast isolates from chenin blanc grapes and grape skins
(South African Society for Enology and Viticulture, 1991)Wild-type killer yeast strains isolated from six South African wineries were identified using classical taxonomic methods. They were further characterized according to their cross-reactions with reference killer yeasts ... -
Characterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from Boza, and comparison of its efficiency to Pediocin PA-1
(PLoS, 2013-08)A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin ... -
A Chromogenic substrate for a β-xylosidase-coupled assay of α-glucuronidase
(Elsevier, 2000-08)-Nitrophenyl 2-(4-O-methyl-α- -glucopyranuronosyl)-β- -xylopyranoside obtained on deesterification of 4-nitrophenyl 2-O-(methyl 4-O-methyl-α- -glucopyranosyluronate)-β- -xylopyranoside (Hirsch et al., Carbohydr. Res. 310, ... -
The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli
(American Society for Microbiology, 2000-05)The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, ... -
Ciprofloxacin-eluting nanofibers inhibits biofilm formation by pseudomonas aeruginosa and a Methicillin-resistant Staphylococcus aureus
(Public Library of Science, 2015-04)Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with hospital- acquired infections and are known to form biofilms. Ciprofloxacin (CIP), which is normally used to treat these infections, is seldom ... -
Cloning of the Bacillus pumilus beta-xylosidase gene (xynB ) and its expression in Saccharomyces cerevisiae
(Springer-Verlag, 1997-07)A genomic DNA library of the bacterium Bacillus pumilus PLS was constructed and the beta-xylosidase gene (xynB) was amplified from a 3-kb genomic DNA fragment with the aid of the polymerase chain reaction technique. The ... -
Co-detection of virulent escherichia coli genes in surface water sources
(Public Library of Science, 2015)McNemar’s test and the Pearson Chi-square were used to assess the co-detection and observed frequency, respectively, for potentially virulent E. coli genes in river water. Conventional multiplex Polymerase Chain Reaction ... -
Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
(Springer-Verlag, 1999-12)The xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the ... -
Combined cell‑surface displayand secretion‑based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
(BioMed Central, 2015-09-26)Background: Engineering Saccharomyces cerevisiae to produce heterologous cellulases is considered as a promising strategy for production of bioethanol from lignocellulose. The production of cellulase is usually pursued ... -
Comparative analysis of solar pasteurization versus solar disinfection for the treatment of harvested rainwater
(BioMed Central, 2016-12-09)Background: Numerous pathogens and opportunistic pathogens have been detected in harvested rainwater. Developing countries, in particular, require time- and cost-effective treatment strategies to improve the quality ... -
Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse
(BioMed Central, 2013-11)Background: The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for ... -
Comparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burden
(PLOS, 2011-12)Introduction: Accurate quantification of mycobacterial load is important for the evaluation of patient infectiousness, disease severity and monitoring treatment response in human and in-vitro laboratory models of disease. ... -
Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains
(Elsevier, 2004-12)β-Glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the β-glucosidases, the genes were fused ... -
Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch
(BMC (part of Springer Nature), 2019-08-20)Background: Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from starchy ... -
Control of malolactic fermentation in wine. a review
(South African Society for Enology and Viticulture, 2004)Malolactic fermentation (MLF) is conducted by lactic acid bacteria (LAB) and refers to the decarboxylation of Lmalate to L-lactate. This secondary fermentation is difficult to control and is mainly driven by Oenococcus ... -
Converting plant biomass to fuels and commodity chemicals in South Africa : a third chapter?
(Academy of Science for South Africa, 2003)THERE HAVE BEEN TWO DISTINCT CHAPTERS in the history of converting cellulosic biomass to fuels and commodity chemicals in South Africa. The first chapter, from the late 1970s to the early 1990s, involved some of the most ... -
Copper-containing anti-biofilm nanofiber scaffolds as a wound dressing material
(Public Library of Science, 2016)Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus ... -
Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) genes
(American Society of Microbiology, 2001-12)The β-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified ...
