Saccharomyces cerevisiae oxidative response evaluation by cyclic voltammetry and gas chromatography-mass spectrometry
Date
2012
Authors
Castro C.C.
Gunning C.
Oliveira C.M.
Couto J.A.
Teixeira J.A.
Martins R.C.
Ferreira A.C.S.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
This study is focused on the evaluation of the impact of Saccharomyces cerevisiae metabolism in the profile of compounds with antioxidant capacity in a synthetic wine during fermentation. A bioanalytical pipeline, which allows for biological systems fingerprinting and sample classification by combining electrochemical features with biochemical background, is proposed. To achieve this objective, alcoholic fermentations of a minimal medium supplemented with phenolic acids were evaluated daily during 11 days, for electrochemical profile, phenolic acids, and the volatile fermentation fraction, using cyclic voltametry, high-performance liquid chromatography-diode array detection, and headspace/solid-phase microextraction/gas chromatography-mass spectrometry (target and nontarget approaches), respectively. It was found that acetic acid, 2-phenylethanol, and isoamyl acetate are compounds with a significative contribution for samples metabolic variability, and the electrochemical features demonstrated redox-potential changes throughout the alcoholic fermentations, showing at the end a similar pattern to normal wines. Moreover, S. cerevisiae had the capacity of producing chlorogenic acid in the supplemented medium fermentation from simple precursors present in the minimal medium. © 2012 American Chemical Society.
Description
Article
Keywords
Bioanalytical methods, chemometrics, electrochemical sensors, mass spectrometry
Citation
Journal of Agricultural and Food Chemistry
60
29
7252
7261
60
29
7252
7261