Analysis of dextrin dextranase from Gluconobacter oxydans

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dc.contributor.advisor Lloyd, J. R. en_ZA
dc.contributor.advisor Kossmann, J. M. en_ZA Van Wyk, Nathan en_ZA
dc.contributor.other Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Institute for Plant Biotechnology. 2008-11-21T06:50:50Z en_ZA 2010-06-01T08:53:46Z 2008-11-21T06:50:50Z en_ZA 2010-06-01T08:53:46Z 2008-12
dc.description Thesis (MSc (Genetics. Institute for Plant Biotechnology (IPB)))--Stellenbosch University, 2008.
dc.description.abstract Dextran is a high value glucose polymer used in medicine and an array of laboratory techniques. It is synthesised by lactic-acid bacteria from sucrose but has also reportedly been produced by Gluconobacter oxydans (G. oxydans) from a range of maltooligosaccharides (MOS) via the action of dextrin dextranase (DDase). In this study the presence of DDase is investigated in two G. oxydans strains (ATCC 621H and ATCC 19357) and shown to be present in the ATCC 19357 strain, but not in the ATCC 621H strain. The enzyme was partially purified from the ATCC 19357 strain, and its kinetic properties investigated. The partially purified protein was also digested with trypsin, and de novo peptide sequences obtained from it. Several attempts were made to obtain the gene coding for the DDase. These include amplifying an open reading frame from the G. oxydans genome coding for a glycosyltransferase with the approximate molecular weight of the DDase, using the peptide sequences obtained from the partially purified protein to design degenerate PCR primers and the production of a genomic DNA library for functional screening in E. coli. None of these approaches led to the successful isolation of the extracellular DDase sequence. en_ZA
dc.language.iso en en_ZA
dc.publisher Stellenbosch : Stellenbosch University
dc.subject Dextrin dextranase en_ZA
dc.subject Gluconobacter oxydans en_ZA
dc.subject Theses -- Plant biotechnology en_ZA
dc.subject Dissertations -- Plant biotechnology en_ZA
dc.title Analysis of dextrin dextranase from Gluconobacter oxydans en_ZA
dc.type Thesis en_ZA
dc.rights.holder Stellenbosch University
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