Analysis of dextrin dextranase from Gluconobacter oxydans

dc.contributor.advisorLloyd, J. R.en_ZA
dc.contributor.advisorKossmann, J. M.en_ZA
dc.contributor.authorVan Wyk, Nathanen_ZA
dc.contributor.otherStellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Institute for Plant Biotechnology.
dc.descriptionThesis (MSc (Genetics. Institute for Plant Biotechnology (IPB)))--Stellenbosch University, 2008.
dc.description.abstractDextran is a high value glucose polymer used in medicine and an array of laboratory techniques. It is synthesised by lactic-acid bacteria from sucrose but has also reportedly been produced by Gluconobacter oxydans (G. oxydans) from a range of maltooligosaccharides (MOS) via the action of dextrin dextranase (DDase). In this study the presence of DDase is investigated in two G. oxydans strains (ATCC 621H and ATCC 19357) and shown to be present in the ATCC 19357 strain, but not in the ATCC 621H strain. The enzyme was partially purified from the ATCC 19357 strain, and its kinetic properties investigated. The partially purified protein was also digested with trypsin, and de novo peptide sequences obtained from it. Several attempts were made to obtain the gene coding for the DDase. These include amplifying an open reading frame from the G. oxydans genome coding for a glycosyltransferase with the approximate molecular weight of the DDase, using the peptide sequences obtained from the partially purified protein to design degenerate PCR primers and the production of a genomic DNA library for functional screening in E. coli. None of these approaches led to the successful isolation of the extracellular DDase sequence.en_ZA
dc.publisherStellenbosch : Stellenbosch University
dc.subjectDextrin dextranaseen_ZA
dc.subjectGluconobacter oxydansen_ZA
dc.subjectTheses -- Plant biotechnologyen_ZA
dc.subjectDissertations -- Plant biotechnologyen_ZA
dc.titleAnalysis of dextrin dextranase from Gluconobacter oxydansen_ZA
dc.rights.holderStellenbosch University

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