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Now showing items 1-11 of 11

Cloning of the Bacillus pumilus β-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae

La Grange D.C. Pretorius I.S. Van Zyl W.H. (1997)

A genomic DNA library of the bacterium Bacillus pumilus PLS was constructed and the β-xylosidase gene (xynB) was amplified from a 3-kb genomic DNA fragment with the aid of the polymerase chain reaction technique. The ...
Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

La Grange D.C. Claeyssens M. Pretorius I.S. Van Zyl W.H. (2000)

The xynB gene encoding the Bacillus pumilus β-xylosidase was expressed separately and jointly with the Trichoderma reesei β-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the ...
Comparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils

Lakay F.M. Botha A. Prior B.A. (2007)

Aim: To establish a rapid, improved soil environmental DNA extraction and purification protocol. Methods and Results: Three different soil DNA isolation and four purification strategies were compared on different soil ...
Effect of organic material and biological amendments on pear tree performance and soil microbial and chemical properties

Van Schoor L. Stassen P.J.C. Botha A. (2012)

The potential of soil for deciduous fruit production may be increased by biological amendment application, especially in pear trees that generally suffer from poor, initial root development. The objective of the study was ...
Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

Southgate V.J. Steyn A.J.C. Pretorius I.S. Van Vuuren H.J.J. (1993)

Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular ...
Isolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish

Lalloo R. Ramchuran S. Ramduth D. Gorgens J. Gardiner N. (2007)

Aims: To isolate, select and evaluate Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. Methods and Results: Natural isolates obtained from mud sediment and Cyprinus ...
Isolation of Alicyclobacillus and the influence of different growth parameters

Witthuhn R.C. Smit Y. Cameron M. Venter P. (2011)

Alicyclobacillus species are thermo-acidophilic, endospore-forming bacteria that are able to survive pasteurisation and have been implicated in a number of spoilage incidents involving acidic foods and beverages. The aim ...
Light and electron microscope assessment of the lytic activity of Bacillus on Microcystis aeruginosa

Gumbo J.R. Cloete T.E. (2011)

During the screening of lytic bacteria, plaques were obtained on Microcystis lawns. In the plaques, at least five distinct morphotypes of bacteria were found. The plumb rod-shaped bacilli were the most abundant and were ...
Microbial content of commercial South African high-moisture dried fruits

Witthuhn R.C. Engelbrecht S. Joubert E. Britz T.J. (2005)

Aim: The aim was to evaluate commercially available South African high-moisture dried fruits (HMDF) for the microbial, moisture and SO2 contents, as well as aw and pH. Methods and Results: The microbial content of commercially ...
Non-indigenous microorganisms in the Antarctic: Assessing the risks

Cowan D.A. Chown S.L. Convey P. Tuffin M. Hughes K. Pointing S. Vincent W.F. (2011)
One-step enzymatic hydrolysis of starch using a recombinant strain of Saccharomyces cerevisiae producing α-amylase, glucoamylase and pullulanase

Janse B.J.H. Pretorius I.S. (1995)

A recombinant strain of Saccharomyces cerevisiae was constructed that contained the genes encoding a bacterial α-amylase (AMY1), a yeast glucoamylase (STA2) and a bacterial pullulanase (pulA). The Bacillus amyloliquefaciens ...