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Department of Microbiology
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Now showing items 1-10 of 10

A Chromogenic substrate for a β-xylosidase-coupled assay of α-glucuronidase

Biely, Peter Hirsch, Jan La Grange, Daniel C. Van Zyl, Willem H. Prior, Bernard A. (Elsevier, 2000-08)

-Nitrophenyl 2-(4-O-methyl-α- -glucopyranuronosyl)-β- -xylopyranoside obtained on deesterification of 4-nitrophenyl 2-O-(methyl 4-O-methyl-α- -glucopyranosyluronate)-β- -xylopyranoside (Hirsch et al., Carbohydr. Res. 310, ...
Cloning of the Bacillus pumilus beta-xylosidase gene (xynB ) and its expression in Saccharomyces cerevisiae

La Grange, Daniel C. Pretorius, Isak S. Van Zyl, Willem H. (Springer-Verlag, 1997-07)

A genomic DNA library of the bacterium Bacillus pumilus PLS was constructed and the beta-xylosidase gene (xynB) was amplified from a 3-kb genomic DNA fragment with the aid of the polymerase chain reaction technique. The ...
Coexpression of the Bacillus pumilus beta-xylosidase (xynB) gene with the Trichoderma reesei beta xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

La Grange, Daniel C. Claeyssens, Mark Pretorius, Isak S. Van Zyl, Willem H. (Springer-Verlag, 1999-12)

The xynB gene encoding the Bacillus pumilus beta-xylosidase was expressed separately and jointly with the Trichoderma reesei beta-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the ...
Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains

Van Rooyen, Ronel Hahn-Hagerdal, Barbel La Grange, Daniel C. Van Zyl, Willem H. (Elsevier, 2004-12)

β-Glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the β-glucosidases, the genes were fused ...
Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) genes

La Grange, Daniel C. Pretorius, Isak S. Claeyssens, Mark Van Zyl, Willem H. (American Society of Microbiology, 2001-12)

The β-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified ...
Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae alpha-amylase in industrial strains of Saccharomyces cerevisiae

Gundllapalli Moses, S. B. Cordero Otero, R. R. La Grange, Daniel C. Van Rensburg, Pierre Pretorius, Isak S. (Kluwer (now part of Springer), 2002-01)

A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae alpha-amylase-encoding gene (LKA1). These transformants ...
Engineering cellulolytic ability into bioprocessing organisms

La Grange, Daniel C. Den Haan, Riaan Van Zyl, Willem H. (Springer-Verlag, 2010-03)

Lignocellulosic biomass is an abundant renewable feedstock for sustainable production of commodities such as biofuels. The main technological barrier that prevents widespread utilization of this resource for production of ...
Enzyme-coupled assay of acetylxylan esterases on monoacetylated 4-nitrophenyl beta-D-xylopyranoside

Biely, Peter Mastihubova, Maria La Grange, Daniel C. Van Zyl, Willem H. Prior, Bernard A. (Elsevier, 2004-03)

Three different monoacetates of 4-nitrophenyl beta-D-xylopyranoside were tested as substrates for beta -xylosidase and for microbial carbohydrate esterases and a series of non-hemicellulolytic esterases. The acetyl group ...
Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae

La Grange, Daniel C. Pretorius, Isak S. Van Zyl, Willem H. (American Society for Microbiology, 1995-04)

The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was ...
Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol

Den Haan, Riaan Mcbride, John E. La Grange, Daniel C. Lynd, Lee R. Van Zyl, Willem H. (Elsevier, 2006-09)

To investigate the possible use of Saccharomyces cerevisiae as a candidate for consolidated bioprocessing (CBP) of cellulose to ethanol, four fungal cellobiohydrolase (CBH) encoding genes (Trichoderma reesei cbh1 and cbh2, ...